用于药物递送的酶促交联原位合成丝素/明胶/磷酸钙水凝胶
Enzymatically Crosslinked In Situ Synthesized Silk/Gelatin/Calcium Phosphate Hydrogels for Drug Delivery.
作者信息
Grava Andra, Egle Karina, Dubnika Arita
机构信息
Rudolfs Cimdins Riga Biomaterials Innovations and Development Centre of RTU, Faculty of Materials Science and Applied Chemistry, Institute of General Chemical Engineering, Riga Technical University, Pulka 3/3, LV-1007 Riga, Latvia.
Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, LV-1658 Riga, Latvia.
出版信息
Materials (Basel). 2021 Nov 25;14(23):7191. doi: 10.3390/ma14237191.
Our research focuses on combining the valuable properties of silk fibroin (SF) and calcium phosphate (CaP). SF is a natural protein with an easily modifiable structure; CaP is a mineral found in the human body. Most of the new age biocomposites lack interaction between organic/inorganic phase, thus SF/CaP composite could not only mimic the natural bone, but could also be used to make drug delivery systems as well, which can ensure both healing and regeneration. CaP was synthesized in situ in SF at different pH values, and then crosslinked with gelatin (G), horseradish peroxide (HRP), and hydrogen peroxide (HO). In addition, dexamethasone phosphate (DEX) was incorporated in the hydrogel and drug delivery kinetics was studied. Hydrogel made at pH 10.0 was found to have the highest gel fraction 110.24%, swelling degree 956.32%, and sustained drug delivery for 72 h. The highest cell viability was observed for the hydrogel, which contained brushite (pH 6)-512.43%.
我们的研究重点是将丝素蛋白（SF）和磷酸钙（CaP）的宝贵特性结合起来。丝素蛋白是一种结构易于修饰的天然蛋白质；磷酸钙是人体中发现的一种矿物质。大多数新时代生物复合材料在有机/无机相之间缺乏相互作用，因此SF/CaP复合材料不仅可以模仿天然骨骼，还可用于制造药物递送系统，既能确保愈合又能促进再生。在不同pH值下，磷酸钙在丝素蛋白中原位合成，然后与明胶（G）、辣根过氧化物酶（HRP）和过氧化氢（HO）交联。此外，将地塞米松磷酸酯（DEX）掺入水凝胶中并研究药物递送动力学。发现在pH 10.0条件下制备的水凝胶具有最高的凝胶分数110.24%、溶胀度956.32%，并能持续释药72小时。观察到含有透钙磷石（pH 6）的水凝胶具有最高的细胞活力——512.43%。
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