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不同品种中国沙棘浆果干燥方法对酚类成分和抗氧化活性的影响。

Impact of Drying Methods on Phenolic Components and Antioxidant Activity of Sea Buckthorn ( L.) Berries from Different Varieties in China.

机构信息

Institute of Medicinal Plant Development, Chinese Academy of Medicinal Sciences & Peking Union Medicinal College, Beijing 100193, China.

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.

出版信息

Molecules. 2021 Nov 26;26(23):7189. doi: 10.3390/molecules26237189.

Abstract

Sea buckthorn berries are rich in bioactive compounds and can be used for medicine and food. The variety and drying method used have an important influence on quality. In this study, different sea buckthorn varieties from China were selected and dried with four common drying methods. The total phenolic content (TPC), total flavonoids content (TFC), contents of 12 phenolic compounds and antioxidant capacity in vitro were analyzed. The results showed that the TPC, TFC and antioxidant activity of two wild sea buckthorn berries were higher than those of three cultivated berries, and for the same varieties, measured chemical contents and antioxidant activity of the freeze-dried fruit were significantly higher than those obtained with three conventional drying methods. In addition, forty-one compounds in sea buckthorn berry were identified by UPLC-PDA-Q/TOF-MS, most of which were isorhamnetin derivatives. Multivariate statistical analysis revealed narcissin and isorhamnetin-3--glucoside varied significantly in sea buckthorn berries of different varieties and with different drying methods; they were potential quality markers. Strong correlations were found between TPC, gallic acid and antioxidant capacity ( < 0.05). The results revealed how components and antioxidant activity varied in different sea buckthorn, which provides a valuable reference for quality control and further development and utilization of sea buckthorn.

摘要

沙棘果实富含生物活性化合物,可用于医药和食品。品种和干燥方法对质量有重要影响。本研究选用中国不同的沙棘品种,采用四种常见的干燥方法进行干燥。分析了总酚含量(TPC)、总黄酮含量(TFC)、12 种酚类化合物含量和体外抗氧化能力。结果表明,两种野生沙棘果实的 TPC、TFC 和抗氧化活性均高于三种栽培品种,对于同一品种,冻干果实的测定化学含量和抗氧化活性明显高于三种常规干燥方法。此外,通过 UPLC-PDA-Q/TOF-MS 鉴定出沙棘浆果中含有 41 种化合物,其中大部分为异鼠李素衍生物。多变量统计分析表明,不同品种和不同干燥方法的沙棘浆果中自恋素和异鼠李素-3--葡萄糖苷差异显著;它们是潜在的质量标志物。TPC、没食子酸和抗氧化能力之间存在很强的相关性(<0.05)。结果揭示了不同沙棘中成分和抗氧化活性的变化,为沙棘的质量控制和进一步开发利用提供了有价值的参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/79b2/8659002/87b7f4e8470e/molecules-26-07189-g001.jpg

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