Federal University of Amazonas, Av. Gal Rodrigo Otávio, 3000, Campus Universitário, Setor Sul, Manaus, AM, 69077-000, Brazil.
Federal University of Roraima, Av. Ene Garcez, 2413, Aeroporto, Boa Vista, RR 69310-000, Brazil.
ScientificWorldJournal. 2021 Dec 3;2021:9929059. doi: 10.1155/2021/9929059. eCollection 2021.
Considering the high biotechnological potential of yeasts associated to edible fruits, a screening for these microorganisms, capable of alcoholic fermentation, was performed in ripe fruits of camu-camu ( Kunth). The fruits were collected from north of Brazilian Amazon, in the floodplain of the Cauamé River. Yeasts were isolated, and fermentation capability was evaluated using Durham tubes. Quantitative assays were performed to calculate ethanol yield (g g), specific growth rate (h), and ethanol productivity (g L·h). Taxonomic identification was performed by ribosomal gene nucleotide sequence analysis by alignment using BLASTN. A total of fifteen yeast colonies were isolated, and three of them presented promising ability to ferment glucose to ethanol. These isolates were identified as , , and . When cultured in broth containing 180 g·L of glucose, CC003 reached 91.7 percent of the maximum theoretical ethanol concentration (84.4 g·L), presenting an ethanol yield and productivity of 0.4688 g·g and 0.781 g·L·h, respectively. These results indicate a promising potential of this isolate for bioprocess applications. This paper is a rare report of with endophytic habit because most of the references indicate it as a human pathogen. Besides this, is a promising fermenter for alcoholic beverages due to its osmotolerance and high ethanol yield. This is the first paper reporting endophytic yeasts associated with fruits of .
考虑到与可食用水果相关的酵母具有很高的生物技术潜力,我们对这些能够进行酒精发酵的微生物进行了筛选,这些微生物来自于坎姆果(Kunth)成熟果实。果实来自于巴西亚马逊北部的考梅内河洪泛区。分离酵母,并使用 Durham 管评估发酵能力。通过定量测定计算乙醇产率(g/g)、比生长速率(h)和乙醇生产速率(g/L·h)。通过使用 BLASTN 对齐核糖体基因核苷酸序列分析进行分类鉴定。总共分离出十五个酵母菌落,其中三个具有将葡萄糖发酵成乙醇的良好能力。这些分离株被鉴定为 、 和 。当在含有 180 g/L 葡萄糖的培养液中培养时,CC003 达到了最大理论乙醇浓度(84.4 g/L)的 91.7%,乙醇产率和生产速率分别为 0.4688 g/g 和 0.781 g/L·h。这些结果表明该分离株在生物过程应用中具有很大的应用潜力。这是一篇关于内生菌的罕见报道,因为大多数参考文献都将其作为人类病原体。此外,由于其耐渗透压和高乙醇产率, 是一种很有前途的酒精饮料发酵剂。这是第一篇报道与坎姆果果实相关的内生酵母的论文。