Calcium pretreatment enhances fluoride reactivity with enamel and dentine.

OBJECTIVE

High fluoride concentration treatments are known to react with enamel and dentine forming calcium fluoride (CaF)-like deposits, but strategies to improve this reactivity beyond increasing fluoride concentration/reducing pH in fluoride treatments have not been explored. Here we investigated the ability of a calcium pre-treatment to improve fluoride reactivity.

DESIGN

In a blind and randomized in vitro study, sound and carious enamel and dentine slabs (n = 11/group) were randomly allocated into one of the following treatments: Deionized water (negative control); 0.05% sodium fluoride (F, positive control); 150 mM calcium lactate solution followed by 0.05% sodium fluoride solution (Ca→F); 150 mM calcium lactate solution premixed with 0.05% sodium fluoride solution (CaF, active control). Alkali-soluble fluoride (representing CaF-like deposits formed on the substrates) was extracted from the slabs using 1 M KOH for 24 h and measured by an ion-specific electrode. Carious slabs were further observed under scanning electron microscopy (SEM). Data were analyzed by two-way ANOVA followed by Tukey test.

RESULTS

The Ca→F treatment enhanced fluoride reactivity with all tested substrates when compared with F alone. Carious substrates had a greater reactivity with F and Ca→F than their respective sound substrates, confirming that increased porosity enhances the reactivity with fluoride. Alkali-soluble fluoride concentration after the CaF treatment did not differ among the different substrates, suggesting this treatment causes only contamination with preformed CaF, which was noted under SEM.

CONCLUSION

A calcium pretreatment enhances the reactivity of fluoride with enamel and dentine.