Metabolomics Study of Flavonoids of on Different Hosts Using UPLC-ESI-MS/MS.

The goal of this study was to identify and compare the main biomarkers of from different hosts. A metabolomics approach utilizing ultra-pressure liquid chromatography coupled with tandem mass spectrometry (UPLC-MS), including cluster analysis, sample correlation analysis and orthogonal partial least squares discriminant analysis, was used to explore the flavonoid metabolites of growing on different hosts. Results: The total flavonoids content (up to 30.08 mg/g) in from (CSG) was significantly higher than that from growth on (CFG) or (CHG) ( < 0.01). There were 23 different metabolites between CSG and CHG, 23 different metabolites between CSG and CFG, and 19 different metabolites between CHG and CFG. The results demonstrated that different hosts exerted a large influence on the metabolites of ; it was found that CSG differed from CFG and CHG in eleven metabolic compounds, ten of which were upregulated and one of which was downregulated. Most of these metabolites derive from compounds contained in the host plant, white mulberry (); many feature potent anti-cancer effects. Differences in host can influence the type and abundance of flavonoids in parasitic plants such as , which is of great significance to researchers seeking to understand the formation mechanism of metabolites. Therefore, attention should be paid to the species of host plant when studying the metabolome. Plants grown on offer the greatest potential for the development of new anti-cancer drugs.