三种非酒精性脂肪性肝病小鼠模型之间肝脏基因表达谱的比较。
Comparison of hepatic gene expression profiles between three mouse models of Nonalcoholic Fatty Liver Disease.
作者信息
Xiang Liping, Jiao Yang, Qian Yiling, Li Yao, Mao Fei, Lu Yan
机构信息
Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, Shanghai 200032, PR China.
Department of Endocrinology and Metabolism, Minhang Branch, Zhongshan Hospital, Central Hospital of Minhang District, Shanghai Minhang Hospital, Fudan University, Shanghai 200032, PR China.
出版信息
Genes Dis. 2021 Feb 27;9(1):201-215. doi: 10.1016/j.gendis.2021.02.008. eCollection 2022 Jan.
Non-alcoholic fatty liver disease (NAFLD) has become the most common chronic liver disorder worldwide. Murine models of NAFLD have been widely used to explore its pathogenesis. In this study, we performed a systematic evaluation of hepatic genome-wide mRNA expression by RNA-Sequencing using three mouse models of NAFLD: leptin receptor deficient db/db mice, high-fat high-sugar diet (HSHF)-induced obese mice, and dexamethasone (DEX)-induced NAFLD mice. As a result, we found both distinct and common pathways in the regulation of lipid metabolism from transcriptomes of three mouse models. Moreover, only a total of 12 differentially expressed genes (DEGs) were commonly detected among all three mouse groups, indicating very little overlap among all three models. Therefore, our results suggest that NAFLD is a heterogeneous disease with highly variable molecular mechanisms.
非酒精性脂肪性肝病（NAFLD）已成为全球最常见的慢性肝脏疾病。NAFLD的小鼠模型已被广泛用于探索其发病机制。在本研究中，我们使用三种NAFLD小鼠模型：瘦素受体缺陷型db/db小鼠、高脂高糖饮食（HSHF）诱导的肥胖小鼠和地塞米松（DEX）诱导的NAFLD小鼠，通过RNA测序对肝脏全基因组mRNA表达进行了系统评估。结果，我们在三种小鼠模型的转录组中发现了脂质代谢调节中既有不同的也有共同的途径。此外，在所有三个小鼠组中总共仅共同检测到12个差异表达基因（DEG），表明所有三个模型之间的重叠非常少。因此，我们的结果表明NAFLD是一种具有高度可变分子机制的异质性疾病。
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