环状 RNA CUL2 通过调节 miR-208a-3p/PPP6C 来调控细胞凋亡和自噬从而影响结直肠癌的发展。
Circular RNA CUL2 regulates the development of colorectal cancer by modulating apoptosis and autophagy via miR-208a-3p/PPP6C.
机构信息
Department of Gastrointestinal Surgery and Anal Diseases, Affiliated Hospital of Weifang Medical College, Weifang, Shandong Province, China.
Basic Medicine Department, Weifang Nursing Vocational College, Weifang, Shandong Province, China.
出版信息
Aging (Albany NY). 2022 Jan 13;14(1):497-508. doi: 10.18632/aging.203827.
AIM
To explore the function of circular RNA CUL2 (circCUL2) in colorectal cancer progression.
METHOD
RT-PCR was carried out to detect the expression of circCUL2 in colorectal cancer tissues and cell lines. Western blot and immunofluorescence were used to determine the level of autophagy. CCK-8, clone formation assay, and EdU staining were used to assess the proliferation ability. Luciferase assay verified the relationship between miR-208a-3p and circCUL2 /PPP6C. The xenograft mouse model was used to confirm the function of circCUL2 .
RESULTS
The expression level of circCUL2 was down-regulated in colorectal cancer tissues and cell lines. Forcing expression of circCUL2 inhibited proliferation ability, induced apoptosis, and autophagy in colorectal cancer cells. Luciferase assay verified that miR-208a-3p could bind with circCUL2/PPP6C. Overexpression of circCUL2 could inhibit cancer progression via targeting the miR-208a-3p/PPP6C signal pathway.
CONCLUSION
CircCUL2 participates in progression via the miR-208a-3p/PPP6C axis in colorectal cancer. CircCUL2 would be an underlying target for the diagnosis and therapy of colorectal cancer.
目的
探索环状 RNA CUL2（circCUL2）在结直肠癌进展中的作用。
方法
采用 RT-PCR 检测结直肠癌组织和细胞系中 circCUL2 的表达。采用 Western blot 和免疫荧光法检测自噬水平。采用 CCK-8、克隆形成实验和 EdU 染色检测增殖能力。荧光素酶实验验证 miR-208a-3p 与 circCUL2/PPP6C 的关系。利用异种移植小鼠模型证实 circCUL2 的功能。
结果
circCUL2 在结直肠癌组织和细胞系中的表达水平下调。强制表达 circCUL2 抑制结直肠癌细胞的增殖能力，诱导细胞凋亡和自噬。荧光素酶实验验证 miR-208a-3p 可与 circCUL2/PPP6C 结合。circCUL2 的过表达可以通过靶向 miR-208a-3p/PPP6C 信号通路抑制癌症进展。
结论
circCUL2 通过结直肠癌中的 miR-208a-3p/PPP6C 轴参与进展。circCUL2 可能成为结直肠癌诊断和治疗的潜在靶点。
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