Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Research Institute of Green Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, Shizuoka, 432-8561, Japan.
Commun Biol. 2022 Jan 19;5(1):68. doi: 10.1038/s42003-022-03014-7.
Bacterial communities associated with aquatic macrophytes largely influence host primary production and nutrient cycling in freshwater environments; however, little is known about how specific bacteria migrate to and proliferate at this unique habitat. Here, we separately identified bacterial genes involved in the initial colonization and overall fitness on plant surface, using the genome-wide transposon sequencing (Tn-seq) of Aquitalea magnusonii H3, a plant growth-promoting bacterium of the floating macrophyte, duckweed. Functional annotation of identified genes indicated that initial colonization efficiency might be simply explained by motility and cell surface structure, while overall fitness was associated with diverse metabolic and regulatory functions. Genes involved in lipopolysaccharides and type-IV pili biosynthesis showed different contributions to colonization and fitness, reflecting their metabolic cost and profound roles in host association. These results provide a comprehensive genetic perspective on aquatic-plant-bacterial interactions, and highlight the potential trade-off between bacterial colonization and proliferation abilities on plant surface.
与水生植物相关的细菌群落在很大程度上影响着淡水环境中宿主的初级生产力和养分循环；然而，人们对于特定细菌如何迁移到这个独特的栖息地并在那里增殖知之甚少。在这里，我们使用全基因组转座子测序（Tn-seq）分别鉴定了与 Aquitalea magnusonii H3 初始定植和整体适应性相关的细菌基因，Aquitalea magnusonii H3 是漂浮植物浮萍的一种促生菌。对鉴定基因的功能注释表明，初始定植效率可能可以简单地用运动性和细胞表面结构来解释，而整体适应性则与多样化的代谢和调控功能有关。参与脂多糖和 IV 型菌毛生物合成的基因对定植和适应性的贡献不同，这反映了它们的代谢成本和在宿主关联中的重要作用。这些结果为水生植物-细菌相互作用提供了全面的遗传视角，并强调了细菌在植物表面定植和增殖能力之间的潜在权衡。
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