Pedobacter segetis sp. nov., a Novel Bacterium Isolated from Soil.

A Gram-negative, motile by gliding, rod-shaped, aerobic bacterium, designated SD-b, was isolated from a soil sample collected on Dokdo Island, South Korea. A polyphasic approach based on phenotypic, phylogenetic, and genomic analyses was used to characterize the new isolate. Phylogenetic analysis of 16S rRNA gene sequence showed that strain SD-b belonged to the family Sphingobacteriaceae and most closely related to Pedobacter psychrophilus P4487A (95.9% similarity). The isolate contained MK-7 as the predominant respiratory quinone; its main polar lipid was phosphatidylethanolamine; and the major fatty acids were summed feature 3 (C ω7c/C ω6c; 32.0%), C iso (19.1%), C iso 3-OH (8.3%), and C (8.2%). The draft genome had a length of 3,842,102 bp with a G+C content of 36.0 mol%, predicting 3282 coding sequences, 3 rRNA genes, 3 ncRNAs, and 36 tRNAs genes. The digital DNA-DNA hybridization and average nucleotide identity values between strain SD-b and P. psychrophilus LMG 29436 were 22.0% and 78.9%, respectively. The results of phenotypic properties, genotypic distinctiveness, and chemotaxonomic features support the discrimination of SD-b from its phylogenetic relatives. Pedobacter segetis sp. nov. is therefore proposed with SD-b (= KCTC 82351 = JCM 34283) as the type strain.