冷等离体氛围微波等离子体的体外抗真菌活性及其与葡萄糖酸氯己定联合应用对厚皮马拉色菌的协同活性。
In vitro antifungal activity of cold atmospheric microwave plasma and synergistic activity against Malassezia pachydermatis when combined with chlorhexidine gluconate.
机构信息
Laboratory of Veterinary Dermatology and the Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea.
出版信息
Vet Med Sci. 2022 Mar;8(2):524-529. doi: 10.1002/vms3.719. Epub 2022 Jan 19.
BACKGROUND
The antifungal efficacy of cold atmospheric microwave plasma (CAMP) against Malassezia pachydermatis has not been to be evaluated.
OBJECTIVE
To examine the antifungal effects of CAMP against M. pachydermatis and its synergistic effects with chlorhexidine gluconate (CHX).
METHODS
A M. pachydermatis isolate was collected from a dog with otitis externa and Malassezia dermatitis at the Seoul National University Veterinary Medical Teaching Hospital. The antifungal effect was determined by applying CAMP to a M. pachydermatis isolate that was incubated for 3 days at 37°C. After 1, 2, 3 and 5 min of application, the efficacy of the plasma treatment was determined according to the number of colony forming units (CFUs). A mixture consisting of inoculum and CHX was applied to evaluate the synergistic effect of the plasma treatment in the same way.
RESULTS
The application of CAMP showed significant antifungal effects against M. pachydermatis. The antifungal effect of CAMP was enhanced by an increased exposure time and output power. The application of CAMP with 0.02% and 0.2% CHX resulted in lower survival rates against M. pachydermatis when compared with its sole application at 1 or 2 min.
CONCLUSIONS
The study findings demonstrate that CAMP has a potential as a new antifungal option for M. pachydermatis and has synergistic antifungal effects with CHX in vitro. Clinical applications for CAMP are necessary to assess the antifungal efficacy for patients.
背景
冷等离体微波等离子体（CAMP）对厚皮马拉色菌的抗真菌效果尚未得到评估。
目的
研究 CAMP 对厚皮马拉色菌的抗真菌作用及其与葡萄糖酸氯己定（CHX）的协同作用。
方法
从首尔国立大学兽医教学医院患有外耳炎和马拉色菌性皮炎的犬中分离出一株厚皮马拉色菌。将 CAMP 应用于在 37°C 孵育 3 天的厚皮马拉色菌分离株，以确定其抗真菌效果。应用等离子体处理 1、2、3 和 5 分钟后，根据菌落形成单位（CFU）的数量确定等离子体处理的效果。将包含接种物和 CHX 的混合物应用于相同的方法来评估等离子体处理的协同作用。
结果
CAMP 对厚皮马拉色菌表现出显著的抗真菌作用。CAMP 的抗真菌作用随着暴露时间和输出功率的增加而增强。与单独应用 1 或 2 分钟相比，应用 0.02%和 0.2% CHX 的 CAMP 导致厚皮马拉色菌的存活率降低。
结论
研究结果表明，CAMP 作为一种新型抗真菌选择，对厚皮马拉色菌具有潜在作用，并与 CHX 在体外具有协同抗真菌作用。需要对 CAMP 进行临床应用评估，以确定其对患者的抗真菌疗效。
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