钒酸盐作为核苷磷酸化酶的一种新底物。
Vanadate as a new substrate for nucleoside phosphorylases.
作者信息
Antipov Alexey N, Okorokova Natalya A, Safonova Tatyana N, Veiko Vladimir P
机构信息
A.N. Bach Institute of Biochemistry, Federal Research Centre of Biotechnology of the Russian Academy of Science, Leninsky Prosp. 33, Build 2, Moscow, 119071, Russia.
Research Centre for Medical Genetics RAMS, Moscow, Russia.
出版信息
J Biol Inorg Chem. 2022 Mar;27(2):221-227. doi: 10.1007/s00775-021-01923-2. Epub 2022 Jan 30.
Orthovanadate was shown to serve as a substrate for nucleoside phosphorylases from Escherichia coli, Shewanella oneidensis, Geobacillus stearothermophilus, and Halomonas chromatireducens AGD 8-3. An exception is thymidine phosphorylase from the extremophilic haloalkaliphilic bacterium Halomonas chromatireducens AGD 8-3, which cannot catalyze the vanadolysis of nucleosides. The kinetic parameters of nucleoside vanadolysis were evaluated.
原钒酸盐被证明可作为大肠杆菌、希瓦氏菌属的奥奈达希瓦氏菌、嗜热栖热放线菌和嗜盐还原菌AGD 8-3中核苷磷酸化酶的底物。嗜盐嗜碱极端微生物嗜盐还原菌AGD 8-3中的胸苷磷酸化酶是个例外，它不能催化核苷的钒解反应。对核苷钒解反应的动力学参数进行了评估。
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