在 ANAMMOX-MFC 处理低 C/N 废水中稳定发电和脱氮的机理。
Mechanism of stable power generation and nitrogen removal in the ANAMMOX-MFC treating low C/N wastewater.
机构信息
Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010031, China; Key Laboratory of Water and Sediment Sciences of Ministry of Education, State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, China.
Key Laboratory of Environmental Pollution Control and Waste Resource Recycle, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010031, China.
出版信息
Chemosphere. 2022 Jun;296:133937. doi: 10.1016/j.chemosphere.2022.133937. Epub 2022 Feb 12.
This study investigated the mechanism of enhanced power generation and nitrogen removal in an ANAMMOX-MFC reactor through subsequent acetate addition. Data showed that nearly 99% total nitrogen removal (≤1 mg L) and 1.41 W m power generation were achieved synchronously under low COD/N (∼1.5) after the subsequent addition of acetate (100 mgCOD·L). The columbic efficiency of the system has increased by 15 times (from 0.64% to 9.48%) after adding acetate. Batch tests showed that the denitrification and ANAMMOX progress occurred synchronously before acetate addition the nitrogen removal rate was accelerated. A distinct shift of bacterial community driven by acetate addition was discovered. The high throughput sequencing analysis indicated acetate addition stimulated the enrichment of denitrifiers, such as Aquimonas, Bradyrhizobium, Thauera, and the potential exoelectrogens changing from Comamonas to Pseudomonas. Functional genes forecasts based on KEGG database and COG database showed that the expressions of TCA functional genes were highly promoted in ANAMMOX-MFC, which demonstrated the enhanced electron transfer pathway driven by acetate addition under low COD/N ratio.
本研究通过后续添加乙酸盐来探究 ANAMMOX-MFC 反应器中增强发电和脱氮的机制。数据表明，在低 COD/N（约 1.5）条件下，后续添加乙酸盐（100mgCOD·L）后，可同步实现近 99%的总氮去除（≤1mgL）和 1.41W·m 的发电。系统的库仑效率增加了 15 倍（从 0.64%增加到 9.48%）。批处理测试表明，在添加乙酸盐之前，反硝化和 ANAMMOX 过程同步发生，从而加速了氮的去除。发现添加乙酸盐会导致细菌群落发生明显变化。高通量测序分析表明，添加乙酸盐刺激了 Aquimonas、Bradyrhizobium、Thauera 等反硝化菌的富集，同时将潜在的电子供体从 Comamonas 改变为 Pseudomonas。基于 KEGG 数据库和 COG 数据库的功能基因预测表明，在 ANAMMOX-MFC 中 TCA 功能基因的表达得到了高度促进，这表明在低 COD/N 比下，乙酸盐的添加驱动了增强的电子传递途径。
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