胆固醇肉芽肿患者 RhoA、ROCK-1 和 ROCK-2 基因表达及多态性
RhoA, ROCK-1, and ROCK-2 Gene Expression and Polymorphisms in Cholesteatoma Patients.
机构信息
Department of Otorhinolaryngology Faculty of Medicine, University of Mersin, Mersin, Turkey.
Department of Pharmacology, Faculty of Medicine, University of Mersin, Mersin, Turkey.
出版信息
J Int Adv Otol. 2021 Nov;17(6):530-535. doi: 10.5152/iao.2021.21132.
BACKGROUND
The aim of this study was to evaluate the gene polymorphism and expressions of Rho-A, ROCK-1, and ROCK-2 in cholesteatoma.
METHODS
In this study, 120 healthy control group patients and 120 cholesteatoma patients were enrolled. Venous blood was taken from all of the cholesteatoma and control group patients. The genotyping of ROCK-1(G/T)rs35996865, ROCK-2(A/C)rs10178332, and Rho-A(A/T)rs2177268 polymorphisms was performed using predesigned TaqMan SNP Genotyping Assays. Assays-on-Demand SNP genotyping kit was used for the realtime polymerase chain reaction. The expression levels of Rho-A(Hs00357608_m1), ROCK-1(Hs01127699_m1), and ROCK-2(Hs00178154_m1) genes were determined.
RESULTS
The expression of Rho-A, ROCK-1, and ROCK-2 was lower in cholesteatoma patients than in the control group. There was no difference in Rho-AAT/TT and ROCK-1GT/TT variation in cholesteatoma patients compared to the control. However, ROCK-2 AC/CC variance was lower in cholesteatoma patients.
CONCLUSION
The expression of Rho-A, ROCK-1, and ROCK-2 genes may be decreased in cholesteatoma. Furthermore, since ROCK-2 AC/CC genotype is also lower in cholesteatoma, having C allele seems to decrease the risk of developing this disease.
背景
本研究旨在评估 Rho-A、ROCK-1 和 ROCK-2 基因多态性和表达在胆脂瘤中的作用。
方法
本研究纳入了 120 例健康对照组患者和 120 例胆脂瘤患者。从所有胆脂瘤和对照组患者中抽取静脉血。采用 TaqMan SNP 基因分型检测试剂盒对 ROCK-1（G/T）rs35996865、ROCK-2（A/C）rs10178332 和 Rho-A（A/T）rs2177268 基因多态性进行基因分型。采用 Assays-on-Demand SNP 基因分型试剂盒进行实时聚合酶链反应。检测 Rho-A（Hs00357608_m1）、ROCK-1（Hs01127699_m1）和 ROCK-2（Hs00178154_m1）基因的表达水平。
结果
胆脂瘤患者 Rho-A、ROCK-1 和 ROCK-2 的表达均低于对照组。与对照组相比，胆脂瘤患者的 Rho-AAT/TT 和 ROCK-1GT/TT 变异无差异。然而，胆脂瘤患者的 ROCK-2 AC/CC 变异较低。
结论
Rho-A、ROCK-1 和 ROCK-2 基因的表达可能在胆脂瘤中降低。此外，由于胆脂瘤中 ROCK-2 AC/CC 基因型也较低，携带 C 等位基因似乎会降低患此病的风险。
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