Du Yanru, Xia Yuxian, Jin Kai
School of Life Sciences, Chongqing University, Chongqing 401331, China.
Chongqing Engineering Research Center for Fungal Insecticide, Chongqing 401331, China.
J Fungi (Basel). 2022 Jan 21;8(2):105. doi: 10.3390/jof8020105.
Entomopathogenic fungi play important roles in the control of populations of agricultural and disease vector pests in nature. The shortcomings of mycoinsecticides for pest management in the field cannot be completely overcome by improving single biocontrol properties of fungi. Therefore, enhancing the biocontrol potential of entomopathogenic fungi in multiple respects by genetic engineering is desirable. Transcription factors are usually involved in various important processes during fungal growth and pathogenesis via regulating a series of genes, and are important candidates for fungal improvement via genetic engineering. Herein, overexpression of , a key transcription factor gene in the cAMP/PKA pathway, improves the biocontrol traits of in multiple respects. When compared with WT, the -overexpression strains exhibit enhanced tolerances to UV-B and heat shock, with increased mean 50% inhibition times by 66.9% and 155.2%, respectively. Advanced conidiation emerged accompanied by increased conidial yield up to 3.89 times after 3-day incubation for the -overexpression strains compared to WT. Furthermore, when compared with WT, the virulence of the -overexpression strains was also increased with the mean 50% lethality times reduced by 21.8% to 23.8%. Taken together, the -overexpression improved the biocontrol potential of in multiple respects. Our results provide insights into the application of key transcription factors for genetic engineering and offer a credible way to further improve the biocontrol potential of entomopathogenic fungi.
昆虫病原真菌在自然界中对农业害虫和病媒害虫种群的控制起着重要作用。通过改善真菌的单一生物防治特性，无法完全克服田间使用真菌杀虫剂进行害虫管理的缺点。因此，通过基因工程从多个方面提高昆虫病原真菌的生物防治潜力是很有必要的。转录因子通常通过调控一系列基因参与真菌生长和致病过程中的各种重要进程，是通过基因工程改良真菌的重要候选对象。在此，cAMP/PKA途径中的关键转录因子基因的过表达在多个方面改善了[真菌名称未给出]的生物防治特性。与野生型相比，[转录因子名称未给出]过表达菌株对UV-B和热休克的耐受性增强，平均50%抑制时间分别增加了66.9%和155.2%。与野生型相比，[转录因子名称未给出]过表达菌株在培养3天后出现了提前产孢，同时分生孢子产量增加至3.89倍。此外，与野生型相比，[转录因子名称未给出]过表达菌株的毒力也有所提高，平均50%致死时间减少了21.8%至23.8%。综上所述，[转录因子名称未给出]过表达在多个方面提高了[真菌名称未给出]的生物防治潜力。我们的研究结果为关键转录因子在基因工程中的应用提供了见解，并为进一步提高昆虫病原真菌的生物防治潜力提供了可靠途径。
