Shigemura Yasutaka, Iwasaki Yu, Sato Yoshio, Kato Tomomi, Seko Takuya, Ishihara Kenji
Department of Nutrition, Faculty of Domestic Science, Tokyo Kasei University, 1-18-1 Kaga, Itabashi-ku, Tokyo 173-8602, Japan.
Seafood Safety and Technology Division, Fisheries Technology Institute, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan.
Foods. 2022 Feb 18;11(4):590. doi: 10.3390/foods11040590.
We examined the absorption of balenine (Bal) in mouse blood after the administration of a high-purity Bal prepared from opah muscle. Using HPLC with phenyl isothiocyanate pre-column derivatization, we successfully isolated imidazole peptides and their constituents. We detected Bal and 3-methylhistidine (3-Me-His) in mouse blood 1 h after the administration of opah-derived Bal. The concentrations of Bal and 3-Me-His significantly increased to 128.27 and 69.09 nmol/mL in plasma, respectively, but were undetectable in control and carnosine (Car)-administrated mice. In contrast, β-alanine and histidine did not increase in mouse plasma 1 h after the administration of Car and opah-derived Bal. The present study is the first report on the absorption of food-derived Bal in mouse blood and serves as a pilot study for future clinical trials.
我们检测了用月鱼肌肉制备的高纯度β-丙氨酰-L-组氨酸（Bal）给药后在小鼠血液中的吸收情况。使用异硫氰酸苯酯柱前衍生化的高效液相色谱法，我们成功分离出咪唑肽及其成分。在给予月鱼来源的Bal 1小时后，我们在小鼠血液中检测到了Bal和3-甲基组氨酸（3-Me-His）。血浆中Bal和3-Me-His的浓度分别显著增加至128.27和69.09 nmol/mL，但在对照小鼠和给予肌肽（Car）的小鼠中未检测到。相比之下，在给予Car和月鱼来源的Bal 1小时后，小鼠血浆中的β-丙氨酸和组氨酸没有增加。本研究是关于食物来源的Bal在小鼠血液中吸收的首次报道，可作为未来临床试验的初步研究。
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