Zheng Yuan, Zhang Jiabin, Meisner Jeffrey, Li Wanjin, Luo Yawen, Wei Fangyu, Wen Liuqing
Carbohydrate-Based Drug Research Center, Shanghai Institute of Materia Media, Chinese Academy of Sciences, Shanghai, 201203, China.
Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Media, Chinese Academy of Sciences, Zhongshan, Guangdong, 528400, China.
Angew Chem Int Ed Engl. 2022 May 9;61(20):e202115696. doi: 10.1002/anie.202115696. Epub 2022 Mar 10.
Glycosylation is catalyzed by glycosyltransferases using sugar nucleotides or occasionally lipid-linked phosphosugars as donors. However, only very few common sugar nucleotides that occur in humans can be obtained readily, while the majority of sugar nucleotides that exist in bacteria, plants, archaea, or viruses cannot be synthesized in sufficient quantities by either enzymatic or chemical synthesis. The limited availability of such rare sugar nucleotides is one of the major obstacles that has greatly hampered progress in glycoscience. Herein we describe a general cofactor-driven cascade conversion strategy for the efficient synthesis of sugar nucleotides. The described strategy allows the large-scale preparation of rare sugar nucleotides from common sugars in high yields and without the need for tedious purification processes.
糖基化由糖基转移酶催化，使用糖核苷酸或偶尔使用脂质连接的磷酸糖作为供体。然而，人类中常见的糖核苷酸只有极少数能够容易地获得，而细菌、植物、古细菌或病毒中存在的大多数糖核苷酸无法通过酶促合成或化学合成大量制备。这些稀有糖核苷酸的有限可用性是严重阻碍糖科学进展的主要障碍之一。在此，我们描述了一种用于高效合成糖核苷酸的通用辅因子驱动级联转化策略。所描述的策略允许从常见糖类高产率地大规模制备稀有糖核苷酸，且无需繁琐的纯化过程。
