State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Nutrients. 2022 Jan 25;14(3):512. doi: 10.3390/nu14030512.
This study was to illustrate the effects of fructooligosaccharide (FOS) on the antioxidant capacity, intestinal barrier function, and microbial community of weanling pigs. Results showed that FOS reduced the incidence of diarrhea (6.5 vs. 10.8%) of pigs (p < 0.05) but did not affect growth performance when compared with the control group. A diet supplemented with FOS increased ileal mRNA expression of occludin (1.7 vs. 1.0), claudin-1 (1.9 vs. 1.0), claudin-2 (1.8 vs. 1.0), and claudin-4 (1.7 vs. 1.0), as well as colonic mRNA expression of ZO-1 (1.6 vs. 1.0), claudin-1 (1.7 vs. 1.0), occludin (1.9 vs. 1.0), and pBD-1 (1.5 vs. 1.0) when compared with the control group (p < 0.05). FOS supplementation improved the anti-oxidase activity and expression of nuclear factor erythroid-2 related factor 2 (Nrf2), and decreased concentrations of D-lactate (3.05 U/L vs. 2.83 U/L) and TNF-α (59.1 pg/mL vs. 48.0 pg/mL) in the serum when compared with the control group (p < 0.05). In addition, FOS increased Sharpea, Megasphaera, and Bacillus populations in the gut when compared with the control group (p < 0.05). Association analysis indicated that mRNA expression of occludin and claudin-1 in the ileal mucosa were correlated positively with populations of Sharpea and Bacillus (p < 0.05). Furthermore, mRNA expression of occludin and claudin-1 in the colonic mucosa were correlated positively with abundances of Sharpea, Lactobocillus, and Bifidobacterium (p < 0.05). In conclusion, FOS activated Nrf2 signaling and increased the expression of specific tight junction proteins, which were associated with reduced diarrhea incidence.
本研究旨在阐明果寡糖（FOS）对断奶仔猪抗氧化能力、肠道屏障功能和微生物群落的影响。结果表明，与对照组相比，FOS 降低了仔猪腹泻的发生率（6.5%对 10.8%）（p<0.05），但对生长性能没有影响。添加 FOS 的日粮增加了回肠中 occludin（1.7 对 1.0）、claudin-1（1.9 对 1.0）、claudin-2（1.8 对 1.0）和 claudin-4（1.7 对 1.0）的 mRNA 表达，以及结肠中 ZO-1（1.6 对 1.0）、claudin-1（1.7 对 1.0）、occludin（1.9 对 1.0）和 pBD-1（1.5 对 1.0）的 mRNA 表达（p<0.05）。与对照组相比，FOS 可改善抗氧化酶活性和核因子红细胞 2 相关因子 2（Nrf2）的表达，降低血清中 D-乳酸（3.05 U/L 对 2.83 U/L）和 TNF-α（59.1 pg/mL 对 48.0 pg/mL）的浓度（p<0.05）。此外，与对照组相比，FOS 增加了肠道中的 Sharpea、Megasphaera 和 Bacillus 种群（p<0.05）。关联分析表明，回肠黏膜中 occludin 和 claudin-1 的 mRNA 表达与 Sharpea 和 Bacillus 的种群呈正相关（p<0.05）。此外，结肠黏膜中 occludin 和 claudin-1 的 mRNA 表达与 Sharpea、Lactobocillus 和 Bifidobacterium 的丰度呈正相关（p<0.05）。综上所述，FOS 激活了 Nrf2 信号通路，增加了特定紧密连接蛋白的表达，这与降低腹泻发生率有关。
