内皮细胞促进雪旺细胞的增殖和迁移。
Endothelial cells promote the proliferation and migration of Schwann cells.
作者信息
Meng De-Hua, Zou Jia-Peng, Xu Qin-Tong, Wang Jia-Yi, Yu Jie-Qin, Yuan Ya, Chen Zeng-Gan, Zhang Ming-He, Jiang Li-Bo, Zhang Jian
机构信息
Department of Orthopedics, Zhongshan Hospital, Fudan University, Shanghai, China.
Department of Orthopedics, The 455th Hospital of Chinese People's Liberation Army, Shanghai, China.
出版信息
Ann Transl Med. 2022 Jan;10(2):78. doi: 10.21037/atm-22-81.
BACKGROUND
After peripheral nerve injury, Schwann cells proliferate and migrate to the injured site, thereby promoting peripheral nerve regeneration. The process is regulated by various factors. Endothelial cells participate in the process via angiogenesis. However, the effects of endothelial cells on Schwann cells are not yet known. The present study sought to evaluate whether endothelial cells accelerate Schwann cell proliferation and migration.
METHODS
We established a co-culture model of rat Schwann cells (RSC96s) and rat aortic endothelial cells (RAOECs), and studied the effects of endothelial cells on Schwann cells by evaluating changes in Schwann cell proliferation and migration and related multiple genes and their protein expressions in the co-culture model.
RESULTS
The results showed that increasing the proportion of endothelial cells in the co-culture model enhanced the proliferation. At days 1 and 3 following the co-culturing, the relative growth rates of the co-cultured cells were 122.87% and 127.37%, respectively, which showed a significant increase in the viability compared to that of the RSC96s (P<0.05). In this process, the expression of Ki67 increased. The migration ability of Schwann cells was also enhanced. The migration capacity of Schwann cells was detected by wound-healing and Transwell assays. The results of the group with 15% of endothelial cells was significantly higher than the results of the other groups (P<0.0001 and P<0.05, respectively). Further, neuregulin 1 and glial fibrillary acidic protein increased the process of Schwann cell migration.
CONCLUSIONS
The results showed that endothelial cells can promote the proliferation and migration of Schwann cells and participate in peripheral nerve regeneration.
背景
周围神经损伤后，施万细胞增殖并迁移至损伤部位，从而促进周围神经再生。该过程受多种因素调控。内皮细胞通过血管生成参与此过程。然而，内皮细胞对施万细胞的影响尚不清楚。本研究旨在评估内皮细胞是否能加速施万细胞的增殖和迁移。
方法
我们建立了大鼠施万细胞（RSC96s）和大鼠主动脉内皮细胞（RAOECs）的共培养模型，并通过评估共培养模型中施万细胞增殖、迁移的变化以及相关多个基因及其蛋白表达，研究内皮细胞对施万细胞的影响。
结果
结果显示，增加共培养模型中内皮细胞的比例可增强增殖。共培养后第1天和第3天，共培养细胞的相对生长率分别为122.87%和127.37%，与RSC96s相比，活力显著增加（P<0.05）。在此过程中，Ki67的表达增加。施万细胞的迁移能力也得到增强。通过伤口愈合和Transwell实验检测施万细胞的迁移能力。含15%内皮细胞组的结果显著高于其他组（分别为P<0.0001和P<0.05）。此外，神经调节蛋白1和胶质纤维酸性蛋白促进了施万细胞的迁移过程。
结论
结果表明，内皮细胞可促进施万细胞的增殖和迁移，并参与周围神经再生。
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