细胞内钙释放对树突特征选择性的区域特异性调节。
Compartment-specific tuning of dendritic feature selectivity by intracellular Ca release.
机构信息
Department of Neuroscience, Columbia University, New York, NY 10027, USA.
Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10027, USA.
出版信息
Science. 2022 Mar 18;375(6586):eabm1670. doi: 10.1126/science.abm1670.
Abstract
Dendritic calcium signaling is central to neural plasticity mechanisms that allow animals to adapt to the environment. Intracellular calcium release (ICR) from the endoplasmic reticulum has long been thought to shape these mechanisms. However, ICR has not been investigated in mammalian neurons in vivo. We combined electroporation of single CA1 pyramidal neurons, simultaneous imaging of dendritic and somatic activity during spatial navigation, optogenetic place field induction, and acute genetic augmentation of ICR cytosolic impact to reveal that ICR supports the establishment of dendritic feature selectivity and shapes integrative properties determining output-level receptive fields. This role for ICR was more prominent in apical than in basal dendrites. Thus, ICR cooperates with circuit-level architecture in vivo to promote the emergence of behaviorally relevant plasticity in a compartment-specific manner.
摘要
树突钙信号是神经可塑性机制的核心，使动物能够适应环境。内质网的细胞内钙释放（ICR）长期以来被认为是塑造这些机制的关键因素。然而，哺乳动物神经元中的 ICR 尚未在体内进行研究。我们结合了单个 CA1 锥体神经元的电穿孔、空间导航过程中树突和体细胞活动的同步成像、光遗传学位置场诱导以及急性遗传增强 ICR 胞质影响，揭示了 ICR 支持树突特征选择性的建立，并塑造了决定输出水平感受野的整合特性。在树突中，这种 ICR 的作用在顶端树突比在基底树突中更为明显。因此，ICR 与体内的电路级结构合作，以特定于隔室的方式促进与行为相关的可塑性的出现。
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