Wang Chong, Zhao Fan, Bai Yun, Li Chunbao, Xu Xinglian, Kristiansen Karsten, Zhou Guanghong
College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Meat Products Processing, Ministry of Agriculture, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing, China.
Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen, Copenhagen, Denmark.
Front Nutr. 2022 Mar 3;9:839364. doi: 10.3389/fnut.2022.839364. eCollection 2022.
We evaluated the possible protective effects of six polyphenols on benzo(a)pyrene (BaP)-induced cytotoxicity in Caco-2 cells. We show that treatment with quinic acid, ferulic acid, homovanillic acid, trolox and BaP decreased cell viability, whereas naringenin and eriodictyol affected viability in a bi-phasic manner with low concentrations decreasing viability whereas higher concentrations increase viability. Co-treatment with 20 μM eriodictyol or naringenin reduced BaP-induced cytotoxicity, including cell apoptosis, cell cycle progression, and oxidative stress. Our results show that the protective effect of eriodictyol was superior to that of naringenin. The potential protective mechanisms of eriodictyol on BaP-induced toxicity were investigated by proteomics. We identified 80 differentially expressed proteins (DEPs) with proteins associated with genetic information processing pathway representing the highest proportion and number of proteins responding to eriodictyol treatment, including key proteins such as RPA2, SNRPA, RAD23B, NUP155 and AARS. Our results provide new knowledge on how polyphenols may prevent BaP-induced carcinogenesis.
我们评估了六种多酚对苯并(a)芘（BaP）诱导的Caco-2细胞毒性的潜在保护作用。我们发现，奎尼酸、阿魏酸、高香草酸、生育三烯酚和BaP处理会降低细胞活力，而柚皮素和圣草酚对活力的影响呈双相性，低浓度时降低活力，而高浓度时则增加活力。用20μM圣草酚或柚皮素共同处理可降低BaP诱导的细胞毒性，包括细胞凋亡、细胞周期进程和氧化应激。我们的结果表明，圣草酚的保护作用优于柚皮素。通过蛋白质组学研究了圣草酚对BaP诱导毒性的潜在保护机制。我们鉴定出80种差异表达蛋白（DEP），其中与遗传信息处理途径相关的蛋白占比和数量最高，是对圣草酚处理有反应的蛋白，包括关键蛋白如RPA2、SNRPA、RAD23B、NUP155和AARS。我们的结果为多酚如何预防BaP诱导的致癌作用提供了新知识。
