Fabrication of chitosan nanofibrous scaffolds based on tannic acid and metal-organic frameworks for hemostatic wound dressing applications.

Here, we developed chitosan (CS)-based nanofibrous scaffold consisting of tannic acid (TA) and zinc-based metal-organic framework (MOF) as a novel antibacterial and hemostatic wound dressing. The effect of MOF content and its incorporation within and onto CS/PVA-TA nanofibrous scaffolds were studied. The morphological characterization of fabricated nanofibrous scaffolds revealed the formation of uniform and bead-free nanofibers with an average diameter between 120 and 150 nm. The uniform and continuous decoration of MOF crystals on nanofibrous scaffold surfaces were confirmed by FESEM. The developed nanofibrous scaffolds exhibit appropriate physicochemical characteristics such as chemical and crystalline structure, surface wettability and swelling, and mechanical properties. It is shown that the incorporation of TA and MOFs greatly enhanced the hemostatic performance of the CS/PVA nanofibrous scaffold by providing rapid liquid absorbability and accelerating the aggregation of coagulation factors and platelets. Furthermore, the results of the MTT assay suggested the good biocompatibility of nanofibrous scaffolds containing MOF nanoparticles. The nanofibrous scaffolds exhibited excellent antibacterial activity against Escherichia coli and Staphylococcus aureus. The disk diffusion antibacterial assay showed that the nanofibrous scaffolds containing TA and MOF could protect wound from bacterial infection. The findings provide new insights to develop a MOF-modified nanofibrous structure with great potential for hemostatic wound dressing application.