Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
The Applied Plant Genomics Laboratory, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Science. 2022 Apr 8;376(6589):180-183. doi: 10.1126/science.abm0717. Epub 2022 Apr 7.
Spike architecture influences grain yield in wheat. We report the map-based cloning of a gene determining the number of spikelet nodes per spike in common wheat. The cloned gene is named and encodes a CONSTANS-like protein that is orthologous to in plant species. Constitutive overexpression of the dominant allele but without the region encoding B-boxes in a common wheat cultivar increases the number of spikelet nodes per spike and produces more tillers and spikes, thereby enhancing grain yield in transgenic plants under field conditions. Allelic variation in results in amino acid substitutions leading to differential protein phosphorylation by the protein kinase K4. The allele is present in emmer wheat but is rare in a global collection of modern wheat cultivars.
穗结构影响小麦的粒重。我们报道了一个控制小麦穗小穗节点数目的基因的图位克隆。该基因被命名为 ，编码一个CONSTANS 样蛋白，与植物物种中的 同源。在普通小麦品种中组成型过表达显性 等位基因，但不包含 B-box 编码区，会增加每个小穗的小穗节点数，并产生更多的分蘖和穗，从而在田间条件下提高转基因植物的粒重。 的等位基因变异导致氨基酸取代，从而导致蛋白激酶 K4 的差异蛋白磷酸化。 等位基因存在于粗山羊草中，但在现代小麦品种的全球收集品系中很少见。
