Key Laboratory of Meat Processing and Quality Control, MOE, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University, Nanjing 210095, China.
School of Food and Wine, Ningxia University, Yinchuan 750021, China.
Nutrients. 2022 Apr 1;14(7):1479. doi: 10.3390/nu14071479.
The bioactive peptides hydrolyzed from bone collagen have been found to possess health-promoting effects by regulating chronic diseases such as arthritis and hypertension. In the current study, the anti-inflammatory effect of bovine bone gelatin peptides (GP) was evaluated in 264.7 macrophages cells and followed by animal trials to investigate their interference on inflammatory cytokines and gut microbiota compositions in dextran sodium sulfate (DSS)-induced C57BL/6 mice. The GP was demonstrated to alleviate the extra secretion of interleukin-6 (IL-6), nitric oxide (NO) and tumor necrosis factor-α(TNF-α) in lipopolysaccharide (LPS)-induced RAW264.7 cells. In DSS-induced colitis mice, the gavage of GP was demonstrated to ameliorate the IBD symptoms of weight loss, hematochezia and inflammatory infiltration in intestinal tissues. In serum, the proinflammatory cytokines (TNF-α,IL-6, MCP-1, IL-1β) were suppressed along with the decreasing effect on toll-like receptor 4 and cyclooxygenase-2 by GP treatment. In the analysis of gut microbiota, the GP was checked to modulate the abundance of , , and . The above results imply that GP could attenuate DSS-induced colitis by suppressing the inflammatory cytokines and regulating the gut microbiota.
从骨胶原中水解得到的生物活性肽被发现具有通过调节关节炎和高血压等慢性疾病来促进健康的作用。在本研究中，评估了牛骨明胶肽（GP）对 264.7 巨噬细胞的抗炎作用，并进行了动物试验，以研究其对葡聚糖硫酸钠（DSS）诱导的 C57BL/6 小鼠炎症细胞因子和肠道微生物组成的干扰。结果表明，GP 减轻了脂多糖（LPS）诱导的 RAW264.7 细胞中白细胞介素-6（IL-6）、一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）的额外分泌。在 DSS 诱导的结肠炎小鼠中，GP 的灌胃可改善 IBD 症状，如体重减轻、血便和肠道组织的炎症浸润。在血清中，促炎细胞因子（TNF-α、IL-6、MCP-1、IL-1β）被抑制，同时 GP 治疗对 Toll 样受体 4 和环氧化酶-2 的抑制作用也被观察到。在肠道微生物组分析中，检查了 GP 对 、 、 和 的丰度的调节作用。上述结果表明，GP 通过抑制炎症细胞因子和调节肠道微生物群来减轻 DSS 诱导的结肠炎。
