School of Automation, Hangzhou Dianzi University, Hangzhou, China.
Engineering for Life Group (EFL), Suzhou, China.
Sci Rep. 2022 Apr 15;12(1):6349. doi: 10.1038/s41598-022-10338-1.
The development of 3D printing has recently attracted significant attention on constructing complex three-dimensional physiological microenvironments. However, it is very challenging to provide a bio-ink with cell-harmless and high mold accuracy during extrusion in 3D printing. To overcome this issue, a technique improving the shear-thinning performance of semi-IPN bio-ink, which is universally applicable to all alginate/gelatin-based materials, was developed. Semi-IPN bio-ink prepared by cyclic heating-cooling treatment in this study can reduce the cell damage without sacrificing the accuracy of the scaffolds for its excellent shear-thinning performance. A more than 15% increase in post-printing Cell viability verified the feasibility of the strategy. Moreover, the bio-ink with low molecular weight and wide molecular weight distribution also promoted a uniform cell distribution and cell proliferation in clusters. Overall, this strategy revealed the effects of molecular parameters of semi-IPN bio-inks on printing performance, and the cell activity was studied and it could be widely applicable to construct the simulated extracellular matrix with various bio-inks.
3D 打印技术在构建复杂三维生理微环境方面引起了广泛关注。然而，在 3D 打印挤出过程中提供细胞无害且高模具精度的生物墨水极具挑战性。为了解决这个问题，开发了一种技术来提高半互穿网络生物墨水的剪切变稀性能，该技术普遍适用于所有基于藻酸盐/明胶的材料。本研究中通过循环加热-冷却处理制备的半互穿网络生物墨水具有出色的剪切变稀性能，可减少细胞损伤，而不会牺牲支架的准确性。打印后细胞活力增加超过 15%验证了该策略的可行性。此外，低分子量和宽分子量分布的生物墨水也促进了细胞在团簇中的均匀分布和增殖。总的来说，该策略揭示了半互穿网络生物墨水的分子参数对打印性能的影响，并研究了细胞活性，它可以广泛适用于用各种生物墨水构建模拟细胞外基质。
