Univ. Grenoble Alpes, CEA, CNRS, Interdisciplinary Research Institute of Grenoble (IRIG), Modeling and Exploration of Materials Laboratory (MEM), 38054 Grenoble, France.
Univ. Grenoble Alpes, CEA, CNRS, Inst. Biol. Struct. IBS, 38044 Grenoble, France.
Chem Rev. 2022 May 25;122(10):9795-9847. doi: 10.1021/acs.chemrev.1c01043. Epub 2022 Apr 21.
Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the investigation of biological systems within their native context, such as lipid membranes, viral capsid assemblies, and cells. However, such ambitious investigations often suffer from low sensitivity due to the presence of significant amounts of other molecular species, which reduces the effective concentration of the biomolecule or interaction of interest. Certain investigations requiring the detection of very low concentration species remain unfeasible even with increasing experimental time for signal averaging. By applying dynamic nuclear polarization (DNP) to overcome the sensitivity challenge, the experimental time required can be reduced by orders of magnitude, broadening the feasible scope of applications for biological solid-state NMR. In this review, we outline strategies commonly adopted for biological applications of DNP, indicate ongoing challenges, and present a comprehensive overview of biological investigations where MAS-DNP has led to unique insights.
固态核磁共振波谱学（ssNMR）与魔角旋转（MAS）相结合，能够在天然环境中研究生物系统，例如脂质膜、病毒衣壳组装体和细胞。然而，由于存在大量其他分子物种，这种雄心勃勃的研究往往会受到低灵敏度的影响，这会降低感兴趣的生物分子或相互作用的有效浓度。即使通过增加信号平均的实验时间，某些需要检测非常低浓度物种的研究仍然是不可行的。通过应用动态核极化（DNP）来克服灵敏度挑战，可以将所需的实验时间减少几个数量级，从而拓宽了生物固态 NMR 的可行应用范围。在这篇综述中，我们概述了常用于生物 DNP 应用的策略，指出了正在面临的挑战，并全面介绍了 MAS-DNP 带来独特见解的生物研究。
