应用脉冲调制射频大气压辉光放电技术降解流动液相中的多西环素。
Application of pulse-modulated radio-frequency atmospheric pressure glow discharge for degradation of doxycycline from a flowing liquid solution.
机构信息
Department of Analytical Chemistry and Chemical Metallurgy, Wroclaw University of Science and Technology, 27 Wybrzeze St. Wyspianskiego, 50-370, Wroclaw, Poland.
Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, 63 Wita Stwosza, 80-308, Gdansk, Poland.
出版信息
Sci Rep. 2022 May 5;12(1):7354. doi: 10.1038/s41598-022-11088-w.
Doxycycline (DOX), an antibiotic commonly used in medicine and veterinary, is frequently detected in natural waterways. Exposition of bacteria to DOX residuals poses a selective pressure leading to a common occurrence of DOX-resistance genetic determinants among microorganisms, including virulent human pathogens. In view of diminishment of the available therapeutic options, we developed a continuous-flow reaction-discharge system generating pulse-modulated radio-frequency atmospheric pressure glow discharge (pm-rf-APGD) intended for DOX removal from liquid solutions. A Design of Experiment and a Response Surface Methodology were implemented in the optimisation procedure. The removal efficiency of DOX equalling 79 ± 4.5% and the resultant degradation products were identified by High-Performance Liquid Chromatography-Diode Array Detection, Liquid Chromatography Quadruple Time of Flight Mass Spectrometry, Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry, total organic carbon, total nitrogen, Attenuated Total Reflectance Furrier Transform-Infrared, and UV/Vis-based methods. The pm-rf-APGD-treated DOX solution due to the generated Reactive Oxygen and Nitrogen Species either lost its antimicrobial properties towards Escherichia coli ATCC25922 or significantly decreased biocidal activities by 37% and 29% in relation to Staphylococcus haemolyticus ATCC29970 and Staphylococcus aureus ATCC25904, respectively. Future implementation of this efficient and eco-friendly antibiotic-degradation technology into wastewater purification systems is predicted.
强力霉素（DOX）是一种常用于医学和兽医的抗生素，经常在天然水道中被检测到。细菌暴露于 DOX 残留物会产生选择性压力，导致微生物中普遍存在 DOX 耐药遗传决定因素，包括有致病性的人类病原体。鉴于可用的治疗选择减少，我们开发了一种连续流动反应-放电系统，产生脉冲调制射频大气压辉光放电（pm-rf-APGD），用于从液体溶液中去除 DOX。在优化过程中实施了实验设计和响应面方法。DOX 的去除效率达到 79±4.5%，并通过高效液相色谱-二极管阵列检测、液相色谱四重飞行时间质谱、超高效液相色谱-串联质谱、总有机碳、总氮、衰减全反射傅里叶变换红外和基于 UV/Vis 的方法鉴定了所得降解产物。由于生成的活性氧和氮物种，pm-rf-APGD 处理的 DOX 溶液要么失去了对大肠杆菌 ATCC25922 的抗菌特性，要么使金黄色葡萄球菌溶血素 ATCC29970 和金黄色葡萄球菌 ATCC25904 的杀菌活性分别显著降低了 37%和 29%。预计未来将这种高效、环保的抗生素降解技术应用于废水净化系统。
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