Wan Xin-Hao, Zhu Wei-Feng, Yang Li-Na, Zhong Ling-Yun, Xia Mei, Li Zhe, Chen Li-Hua, Zang Zhen-Zhong, Guan Yong-Mei
Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine Nanchang 330004, China.
School of Pharmacy, Jiangxi University of Chinese Medicine Nanchang 330004, China.
Zhongguo Zhong Yao Za Zhi. 2022 May;47(9):2430-2439. doi: 10.19540/j.cnki.cjcmm.20211217.302.
A total of 15 batches of the substance reference of Guizhi Jia Gegen Decoction(GZGGD) were prepared and the characteristic fingerprints of them were established. Furthermore, the similarity of the fingerprints and peak attributes were explored. The extraction rate, and the content and the transfer rate ranges of the index components, puerarin, paeoniflorin, liquiritin, and ammonium glycyrrhizate were determined for the analysis of the quality value transfer. The result demonstrated that the fingerprints of the 15 batches of the samples showed high similarity(>0.99). A total of 15 characteristic peaks were identified from the fingerprints, with 10 for Puerariae Lobatae Radix, 1 for Cinnamomi Ramulus, 2 for Paeoniae Radix Alba, and 2 for Glycyrrhizae Radix et Rhizoma. The content of puerarin was 11.05-18.35 mg·g(-1) and the average transfer rate was 21.27%-39.49%. The corresponding figures were 7.95-10.90 mg·g(-1) and 23.28%-43.23% for paeoniflorin, 3.25-4.95 mg·g(-1) and 32.31%-61.27% for ammonium glycyrrhizate, and 3.65-5.80 mg·g(-1) and 14.57%-27.05% for liquiritin. The extraction rate of the 15 batches of samples was in the range of 16.85%-21.78%. In this paper, the quality value transfer of the substance reference of GZGGD was analyzed based on characteristic fingerprint, content of index components, and the extraction rate. This study is expected to lay a basis for the quality control and further development of GZGGD.
制备了15批桂枝加葛根汤物质基准，并建立了其特征指纹图谱。此外，还对指纹图谱的相似度及峰归属进行了探究。测定了指标成分葛根素、芍药苷、甘草苷及甘草酸铵的提取率、含量及转移率范围，以分析质量值传递情况。结果表明，15批样品的指纹图谱相似度较高（>0.99）。从指纹图谱中鉴定出15个特征峰，其中葛根10个、桂枝1个、白芍2个、甘草2个。葛根素含量为11.05 - 18.35 mg·g⁻¹，平均转移率为21.27% - 39.49%。芍药苷相应的数据为7.95 - 10.90 mg·g⁻¹和23.28% - 43.23%，甘草酸铵为3.25 - 4.95 mg·g⁻¹和32.31% - 61.27%，甘草苷为3.65 - 5.80 mg·g⁻¹和14.57% - 27.05%。15批样品的提取率在16.85% - 21.78%范围内。本文基于特征指纹图谱、指标成分含量及提取率对桂枝加葛根汤物质基准的质量值传递进行了分析。本研究有望为桂枝加葛根汤的质量控制和进一步开发奠定基础。
