基于铂@银纳米花的新检测方法对痛风患者血清中尿酸的超灵敏检测
Ultrasensitive detection of uric acid in serum of patients with gout by a new assay based on Pt@Ag nanoflowers.
作者信息
Wang Xue, Chen Shujun, Tang Xiaomin, Lin Daiqin, Qiu Ping
机构信息
Department of Chemistry, Nanchang University Nanchang 330031 China
The Fourth Affiliated Hospital of Nanchang University Nanchang 330003 China.
出版信息
RSC Adv. 2019 Nov 11;9(63):36578-36585. doi: 10.1039/c9ra06481h.
A ultrasensitive assay for the determination of uric acid (UA) based on Pt@Ag nanoflowers (Pt@Ag NFs) was constructed. HO was formed by the reaction of uricase and UA and produced the hydroxyl radical (˙OH). The system was catalyzed by Pt@Ag NFs to change the color of 3,3',5,5'-tetramethylbenzidine (TMB) from colorless to blue, and the morphology and chemical properties of Pt@Ag NFs were characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. Under the optimized conditions, a linear relationship between the absorbance and UA concentration was in the range of 0.5-150 μM ( = 0.995) with a limit of detection of 0.3 μM (S/N = 3). The method can be applied to detection of UA in actual samples with satisfactory results. The proposed assay was successfully applied to the detection of UA in human serum with recoveries over 96.8%. Thus, these results imply that the UA assay provides an effective tool in fast clinical analysis of gout.
构建了一种基于铂@银纳米花（Pt@Ag NFs）的超灵敏尿酸（UA）检测方法。尿酸酶与尿酸反应生成H₂O₂并产生羟基自由基（˙OH）。该体系在Pt@Ag NFs催化下使3,3',5,5'-四甲基联苯胺（TMB）颜色由无色变为蓝色，通过透射电子显微镜和X射线光电子能谱对Pt@Ag NFs的形貌和化学性质进行了表征。在优化条件下，吸光度与UA浓度在0.5 - 150 μM范围内呈线性关系（R² = 0.995），检测限为0.3 μM（S/N = 3）。该方法可应用于实际样品中UA的检测，结果令人满意。所提出的检测方法成功应用于人体血清中UA的检测，回收率超过96.8%。因此，这些结果表明该UA检测方法为痛风的快速临床分析提供了一种有效的工具。
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