Liu Hengzhao, Ye Hang, Zhao Peng
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, China.
Mitochondrial DNA B Resour. 2022 May 4;7(5):744-746. doi: 10.1080/23802359.2022.2070041. eCollection 2022.
'GZSLKY Youyou'. is a new cultivar of with highly ornamental and horticultural value developed by crossing female parent T. Tang & F. T. Wang 1940 and male parent T. Tang & F. T. Wang 1940. In this study, the complete chloroplast genome of the cultivar has been reconstructed from the Illumina sequencing data. The circular genome was 160,503 bp in size, containing a large single-copy region (91,582 bp), a small single-copy region (3,215 bp) and a pair of IR regions (each one 32,853 bp). The overall GC contents of the chloroplast genome was 36.20%. The chloroplast genome contained 122 genes, including 76 protein coding genes, 38 tRNA genes and 8 rRNA genes. A maximum likelihood (ML) phylogenetic tree showed that the cultivar 'GZSLKY Youyou' was clustered into the same clade with its parents and was closest related to , reflecting a maternal inheritance of chloroplasts. This complete chloroplast genome resource could be further used for genomic studies, phylogenetic analyses, and genetic engineering and breeding of the genus .
‘GZSLKY Youyou’是通过母本唐菖蒲(T. Tang & F. T. Wang 1940)与父本唐菖蒲(T. Tang & F. T. Wang 1940)杂交培育出的具有较高观赏和园艺价值的新品种。在本研究中,已从Illumina测序数据重建了该品种的完整叶绿体基因组。环状基因组大小为160,503 bp,包含一个大单拷贝区域(91,582 bp)、一个小单拷贝区域(3,215 bp)和一对IR区域(每个32,853 bp)。叶绿体基因组的总体GC含量为36.20%。叶绿体基因组包含122个基因,包括76个蛋白质编码基因、38个tRNA基因和8个rRNA基因。最大似然(ML)系统发育树表明,品种‘GZSLKY Youyou’与其亲本聚在同一分支,且与[此处原文缺失相关品种名]关系最密切,反映了叶绿体的母系遗传。该完整叶绿体基因组资源可进一步用于该属的基因组研究、系统发育分析以及基因工程和育种。