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“桂紫丝荔幽幽”(×. 的杂交种)叶绿体全基因组的系统发育关系及特征分析

Phylogenetic relationships and characterization of the complete chloroplast genome of 'GZSLKY Youyou', a hybrid of × .

作者信息

Liu Hengzhao, Ye Hang, Zhao Peng

机构信息

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, China.

出版信息

Mitochondrial DNA B Resour. 2022 May 4;7(5):744-746. doi: 10.1080/23802359.2022.2070041. eCollection 2022.

Abstract

'GZSLKY Youyou'. is a new cultivar of with highly ornamental and horticultural value developed by crossing female parent T. Tang & F. T. Wang 1940 and male parent T. Tang & F. T. Wang 1940. In this study, the complete chloroplast genome of the cultivar has been reconstructed from the Illumina sequencing data. The circular genome was 160,503 bp in size, containing a large single-copy region (91,582 bp), a small single-copy region (3,215 bp) and a pair of IR regions (each one 32,853 bp). The overall GC contents of the chloroplast genome was 36.20%. The chloroplast genome contained 122 genes, including 76 protein coding genes, 38 tRNA genes and 8 rRNA genes. A maximum likelihood (ML) phylogenetic tree showed that the cultivar 'GZSLKY Youyou' was clustered into the same clade with its parents and was closest related to , reflecting a maternal inheritance of chloroplasts. This complete chloroplast genome resource could be further used for genomic studies, phylogenetic analyses, and genetic engineering and breeding of the genus .

摘要

‘GZSLKY Youyou’是通过母本唐菖蒲(T. Tang & F. T. Wang 1940)与父本唐菖蒲(T. Tang & F. T. Wang 1940)杂交培育出的具有较高观赏和园艺价值的新品种。在本研究中,已从Illumina测序数据重建了该品种的完整叶绿体基因组。环状基因组大小为160,503 bp,包含一个大单拷贝区域(91,582 bp)、一个小单拷贝区域(3,215 bp)和一对IR区域(每个32,853 bp)。叶绿体基因组的总体GC含量为36.20%。叶绿体基因组包含122个基因,包括76个蛋白质编码基因、38个tRNA基因和8个rRNA基因。最大似然(ML)系统发育树表明,品种‘GZSLKY Youyou’与其亲本聚在同一分支,且与[此处原文缺失相关品种名]关系最密切,反映了叶绿体的母系遗传。该完整叶绿体基因组资源可进一步用于该属的基因组研究、系统发育分析以及基因工程和育种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e63f/9090421/6c4729363976/TMDN_A_2070041_F0001_B.jpg

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