固有自然杀伤 T 细胞及其亚型可能在子宫内膜异位症的发病机制中起作用。
Invariant Natural Killer T-cells and their subtypes may play a role in the pathogenesis of endometriosis.
机构信息
Endometriosis Section, Divisão de Clínica Ginecológica, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil; Gynecology and Obstetrics Department, Faculdade de Medicina da Universidade de Brasília (UnB), Campus Universitário Darcy Ribeiro, Brasília, DF, Brazil.
Endometriosis Section, Divisão de Clínica Ginecológica, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo (HCFMUSP), São Paulo, SP, Brazil; Gynecologic Division, BP ‒ A Beneficência Portuguesa de São Paulo, São Paulo, SP, Brazil.
出版信息
Clinics (Sao Paulo). 2022 May 13;77:100032. doi: 10.1016/j.clinsp.2022.100032. eCollection 2022.
OBJECTIVE
To evaluate the frequencies of iNKT cells and their subsets in patients with deep endometriosis.
METHODS
A case-control study was conducted between 2013 and 2015, with 73 patients distributed into two groups: 47 women with a histological diagnosis of endometriosis and 26 controls. Peripheral blood, endometriosis lesions, and healthy peritoneal samples were collected on the day of surgery to determine the frequencies of iNKT cells and subtypes via flow cytometry analysis.
RESULTS
The authors observed a lower number of iNKT (p = 0.01) and Double-Negative (DN) iNKT cells (p = 0.02) in the blood of patients with endometriosis than in the control group. The number of DN iNKT IL-17 cells in the secretory phase was lower in the endometriosis group (p = 0.049). There was an increase in the secretion of IL-17 by CD4 iNKT cells in the blood of patients with endometriosis and severe dysmenorrhea (p = 0.038), and severe acyclic pelvic pain (p = 0.048). Patients with severe dysmenorrhea also had a decreased number of CD4 CCR7 cells (p = 0.022).
CONCLUSION
The decreased number of total iNKT and DN iNKT cells in patients with endometriosis suggests that iNKT cells play a role in the pathogenesis of endometriosis and can be used to develop new diagnostic and therapeutic agents.
目的
评估深部子宫内膜异位症患者 iNKT 细胞及其亚群的频率。
方法
本病例对照研究于 2013 年至 2015 年进行，将 73 名患者分为两组：47 名组织学诊断为子宫内膜异位症的女性和 26 名对照。在手术当天采集外周血、子宫内膜异位症病变和健康腹膜样本，通过流式细胞术分析确定 iNKT 细胞及其亚型的频率。
结果
与对照组相比，作者观察到子宫内膜异位症患者血液中 iNKT（p=0.01）和双阴性（DN）iNKT 细胞（p=0.02）数量较低。子宫内膜异位症组分泌期 DN iNKT IL-17 细胞数量较低（p=0.049）。子宫内膜异位症和严重痛经患者血液中 CD4 iNKT 细胞分泌的 IL-17 增加（p=0.038），非周期性盆腔疼痛严重患者（p=0.048）也增加。严重痛经患者的 CD4 CCR7 细胞数量也减少（p=0.022）。
结论
子宫内膜异位症患者总 iNKT 和 DN iNKT 细胞数量减少表明 iNKT 细胞在子宫内膜异位症的发病机制中起作用，并可用于开发新的诊断和治疗药物。
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