Enhanced co-production of medium-chain-length polyhydroxyalkanoates and phenazines from crude glycerol by high cell density cultivation of Pseudomonas chlororaphis in membrane bioreactor.

Enhanced co-production of medium-chain-length polyhydroxyalkanoates (mcl-PHA) and extracellular phenazines was assessed through a high cell density cultivation of Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) in a membrane bioreactor using crude glycerol as a fermentative substrate. A maximum dry cell weight (DCW) of 59.25 ± 0.31 g/L was achieved at 90 h of cultivation with a maximum mcl-PHA and extracellular phenazines concentrations of respectively 19.05 ± 0.04 g/L (32.16% of DCW) and 79.42 ± 0.35 mg/L. mcl-PHA concentration achieved through cell retention culture was 28.43-folds higher than that obtained by batch culture. Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and H nuclear magnetic resonance analysis identified the produced PHA as a mcl-PHA copolymer of 3-hydroxyhexanoate (0.68%), 3-hydroxyoctanoate (7.76%), 3-hydroxydecanoate (49.18%), 3-hydroxydodecanoate (4.89%), and 3-hydroxytetradecanoate (37.50%). The mcl-PHA exhibited a highly amorphous structure with low crystallinity index (4.19%) and high thermal stability. This is the first report on the enhanced co-production of mcl-PHA and phenazines in a membrane bioreactor.