Hosseinpour-Niazi Somayeh, Mirmiran Parvin, Hadaegh Farzad, Daneshpour Maryam S, Hedayati Mehdi, Azizi Fereidoun
Nutrition and Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, No. 24, A'rabi St., Yeman Av., Velenjak, Tehran, 19395-4763, Iran.
Prevention of Metabolic Disorders Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Nutr Metab (Lond). 2022 May 18;19(1):35. doi: 10.1186/s12986-022-00671-7.
This randomized controlled trial aimed to investigate the effects of replacing red meat with legumes in the dietary approach to stop hypertension (DASH) diet on inflammatory markers over 16 weeks in overweight and obese individuals with type 2 diabetes. Also, the modulatory effects of TCF7L2 rs7903146 variant on this effect were assessed.
In this trial, 300 participants with type 2 diabetes, aged 30-65 years with an identified TCF7L2 rs7903146 genotype, were studied. The participants were randomly assigned to the DASH diet or the legume-based DASH diet over 16 weeks. In the DASH diet group, the participants were instructed to follow the standard DASH diet. The legume-based DASH diet was similar to the standard DASH diet, with the exception that one serving of red meat was replaced with one serving of legumes at least five days a week. At the beginning of the study and 16-week follow-up, venous blood samples were collected from all participants who fasted for 12-14 h overnight. The serum concentration of High-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was measured using an enzyme-linked immunosorbent assay (ELISA) kit. Also, the serum malondialdehyde (MDA) concentration was assessed based on a colorimetric method using a commercial kit. The primary outcome was the difference in hs-CRP changes between the diets. A secondary outcomes was the difference in IL-6, TNF-α, and MDA between the groups among total population and based on TCF7L2 rs7903146 risk allele (CT + TT) and non-risk allele (CC) separately.
The hs-CRP level reduced in the legume-based DASH diet group as compared to the DASH diet group in the 16-week follow-up group. The levels of TNF-α, IL-6, and MDA reduced after the legume-based DASH diet relative to the DASH diet. Reduction of inflammatory markers was observed in both carriers of rs7903146 risk allele and non-risk allele.
Substituting one serving of red meat with one serving of legumes in DASH diet, at least five days a week, could improve the hs-CRP, TNF-α, IL-6, and MDA in participants with type 2 diabetes regardless of having rs7903146 risk or non-risk allele. Trial registration IRCT, IRCT20090203001640N17.
这项随机对照试验旨在研究在以饮食方法阻止高血压（DASH）饮食中，用豆类替代红肉对超重和肥胖的2型糖尿病患者16周内炎症标志物的影响。此外，还评估了TCF7L2 rs7903146变异对此效果的调节作用。
在该试验中，对300名年龄在30 - 65岁、已确定TCF7L2 rs7903146基因型的2型糖尿病患者进行了研究。参与者被随机分配接受16周的DASH饮食或基于豆类的DASH饮食。在DASH饮食组中，指导参与者遵循标准的DASH饮食。基于豆类的DASH饮食与标准DASH饮食相似，不同之处在于每周至少五天用一份豆类替代一份红肉。在研究开始时和16周随访时，从所有过夜禁食12 - 14小时的参与者中采集静脉血样。使用酶联免疫吸附测定（ELISA）试剂盒测量高敏C反应蛋白（hs-CRP）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的血清浓度。此外，使用商用试剂盒基于比色法评估血清丙二醛（MDA）浓度。主要结局是两种饮食之间hs-CRP变化的差异。次要结局是总人群中以及分别基于TCF7L2 rs7903146风险等位基因（CT + TT）和非风险等位基因（CC）的两组之间IL-6、TNF-α和MDA的差异。
在16周随访组中，与DASH饮食组相比，基于豆类的DASH饮食组的hs-CRP水平降低。与DASH饮食相比，基于豆类的DASH饮食后TNF-α、IL-6和MDA的水平降低。在rs7903146风险等位基因携带者和非风险等位基因携带者中均观察到炎症标志物的降低。
在DASH饮食中，每周至少五天用一份豆类替代一份红肉，无论参与者有无rs7903146风险等位基因，均可改善2型糖尿病患者的hs-CRP、TNF-α、IL-6和MDA水平。试验注册号IRCT，IRCT20090203001640N17。
