Effects of Low-Carbohydrate Diet and Exercise Training on Gut Microbiota.

OBJECTIVE

This study was aimed to evaluate the effects of low-carbohydrate diet (LC) and incorporated high-intensity interval training (HIIT) or moderate-intensity continuous training (MICT) on gut microbiota, and the associations between changes in gut microbiota and cardiometabolic health-related profiles.

METHODS

Fifty overweight/obese Chinese females (age 22.2 ± 3.3 years, body mass index 25.1 ± 3.1 kg/m) were randomized to the groups of LC, LC and HIIT (LC-HIIT, 10 repetitions of 6-s sprints and 9-s rest), and LC and MICT group (LC-MICT, cycling at 50-60% V̇O for 30 min). The LC-HIIT and LC-MICT experienced 20 training sessions over 4 weeks.

RESULTS

The 4-week LC intervention with/without additional training failed to change the Shannon, Chao 1, and Simpson indexes ( > 0.05), LC increased genus, and LC-HIIT reduced genus after intervention ( < 0.05). Groups with extra exercise training increased short-chain fatty acid-producing genus ( < 0.05) and reduced type 2 diabetes-related genus ( < 0.05) compared to LC. ( = -0.335) and ( = 0.334) were associated with changes in body composition ( < 0.05). Changes in , , and genera were positively associated with blood pressure (BP) changes ( = 0.392-0.445, < 0.05), whereas the changes in , , and genera were negatively associated with BP changes ( = -0.567 to -0.362, < 0.05).

CONCLUSION

LC intervention did not change the α-diversity and overall structure of gut microbiota. Combining LC with exercise training may have additional benefits on gut physiology. Specific microbial genera were associated with LC- and exercise-induced regulation of cardiometabolic health.