一种新型大肠杆菌噬菌体 vB_EcoM-RPN242 的特性和全基因组分析。
Characterization and complete genome analysis of a novel Escherichia phage, vB_EcoM-RPN242.
机构信息
Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom, 73000, Thailand.
Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen, Nakhonpathom, Thailand.
出版信息
Arch Virol. 2022 Aug;167(8):1675-1679. doi: 10.1007/s00705-022-05479-7. Epub 2022 May 22.
The novel Escherichia phage vB_EcoM-RPN242 was isolated using a strain of Escherichia coli originating from a diarrheic piglet as a host. The phage was able to form plaques on the E. coli lawn at 15-45 °C. Moreover, it was stable over a wide pH (4-10) and temperature (4-70 °C) range. The vB_EcoM-RPN242 genome was found to be a linear, double-stranded DNA consisting of 154,840 base pairs. There were 195 protein-encoding genes and two tRNAs detected in the genome; however, no genes associated with virulence, toxins or antimicrobial resistance were found. According to overall nucleotide sequence comparisons, vB_EcoM-RPN242 possibly represents a new species in the genus Agtrevirus.
新型大肠杆菌噬菌体 vB_EcoM-RPN242 是利用一株来源于腹泻仔猪的大肠杆菌作为宿主分离得到的。该噬菌体能够在 15-45°C 的大肠杆菌菌苔上形成噬菌斑。此外，它在较宽的 pH（4-10）和温度（4-70°C）范围内稳定。vB_EcoM-RPN242 基因组是一条线性双链 DNA，由 154840 个碱基对组成。在基因组中检测到 195 个蛋白编码基因和两个 tRNA；然而，没有发现与毒力、毒素或抗药性相关的基因。根据总体核苷酸序列比较，vB_EcoM-RPN242 可能代表 Agtrevirus 属中的一个新种。
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