Department of Prosthodontics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Department of Oral and Maxillofacial Surgery, Henan Provincial Stomatological Hospital, Zhengzhou, Henan, China.
Bioengineered. 2022 May;13(5):13055-13069. doi: 10.1080/21655979.2022.2074106.
Oral squamous cell carcinoma (OSCC) is a common cancer with high recurrence, metastasis rates and poor prognosis. Circular RNAs (circRNAs) take part in regulating OSCC. Herein, we examined the role of circ_0008068 in OSCC. The circ_0008068, Katanin p60 ATPase-containing subunit A-like 1 (KATNAL1) mRNA, microRNA-153-3p (miR-153-3p) and acylgycerol kinase (AGK) contents were indicated by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Moreover, in vitro and in vivo assays were conducted to scrutinize the effects of circ_0008068 on OSCC. Additionally, the contact between miR-153-3p and circ_0008068 or AGK was assessed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Thereafter, we found that the appearance of circ_0008068 and AGK was increased, and miR-153-3p content was diminished in OSCC. Circ_0008068 lack subdued cell proliferation, migration, invasion, tube formation and glycolysis metabolism, but stimulated cell apoptosis in OSCC. In addition, circ_0008068 bound to miR-153-3p to modulate the expression of its target AGK. Besides, miR-153-3p was validated to act as a tumor suppressor in OSCC tumorigenesis by suppressing AGK. Additionally, circ_0008068 knockdown also attenuated tumor growth in nude mice. In all, circ_0008068 expedited the growth of OSCC by miR-153-3p/AGK axis. OSCC: Oral squamous cell carcinoma; AGK: Acylgycerol kinase; CircRNA: Circular RNA; KATNAL1: Katanin p60 ATPase-containing subunit A-like 1; qRT-PCR: Quantitative real-time polymerase chain reaction; miRNAs/miRs: MicroRNAs; RIP: RNA immunoprecipitation; 3'UTR3': -untranslated region; HK2: Hexokinase 2; LDHA Lactate dehydrogenase A; IHC: Immunohistochemistry; CCK8: Cell counting kit-8; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
口腔鳞状细胞癌（OSCC）是一种常见的癌症，具有高复发率、转移率和预后不良的特点。环状 RNA（circRNA）参与调节 OSCC。在此，我们研究了 circ_0008068 在 OSCC 中的作用。通过定量实时聚合酶链反应（qRT-PCR）和 Western blot 检测 circ_0008068、Katanin p60 ATPase 含有亚基 A 样 1（KATNAL1）mRNA、微小 RNA-153-3p（miR-153-3p）和酰基甘油激酶（AGK）的含量。此外，还进行了体外和体内实验来研究 circ_0008068 对 OSCC 的影响。此外，通过双荧光素酶报告基因检测和 RNA 免疫沉淀（RIP）实验评估了 miR-153-3p 与 circ_0008068 或 AGK 之间的相互作用。随后，我们发现 circ_0008068 和 AGK 的表达增加，而 miR-153-3p 的含量在 OSCC 中减少。circ_0008068 缺失可抑制 OSCC 细胞的增殖、迁移、侵袭、管形成和糖酵解代谢，但促进细胞凋亡。此外，circ_0008068 与 miR-153-3p 结合，调节其靶基因 AGK 的表达。此外，miR-153-3p 通过抑制 AGK 在 OSCC 肿瘤发生中发挥肿瘤抑制作用。此外，circ_0008068 敲低也可抑制裸鼠肿瘤生长。总之，circ_0008068 通过 miR-153-3p/AGK 轴促进 OSCC 的生长。
