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普鲁兰酶酶解对橡子粉丝质构的影响及其对品质的影响机制。

Effects of pullulanase enzymatic hydrolysis on the textural of acorn vermicelli and its influencing mechanism on the quality.

机构信息

Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.

Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha, PR China.

出版信息

Food Res Int. 2022 Jun;156:111294. doi: 10.1016/j.foodres.2022.111294. Epub 2022 Apr 25.

Abstract

The effect of pullulanase enzymatic hydrolysis time on the textural properties of acorn vermicelli was investigated by texture analyzer. And the influencing mechanism was revealed by exploring the physicochemical properties of acorn starch under the optimum enzymatic hydrolysis time by texture analyzer, scanning electron microscopy, X-ray diffraction and brabender viscograph. After acorn starch was hydrolyzed by pullulanase for 14 h, acorn vermicelli had excellent textural properties. In addition, the enzymatic hydrolysis transformed the acorn starch from spherical particles with smooth surface to polygonal particles with rough surface, as well as transformed the crystal structure of acorn starch from C-type to B-type. Compared with native acorn starch, enzyme hydrolyzed acorn starch had higher amylose content, better freeze-thaw stability, lower swelling power and, breakdown viscosity, stronger gel strength and, higher light transmittance. These excellent properties contributed to the exceptional textural properties and quality of acorn vermicelli. The results of this study may provide valuable information on the preparation of acorn vermicelli.

摘要

利用质构仪研究了普鲁兰酶酶解时间对橡实粉丝质构特性的影响,并通过质构仪、扫描电子显微镜、X 射线衍射和布拉班德黏度计探索了最佳酶解时间下橡实淀粉的理化特性,揭示了其影响机制。在经过 14 h 的普鲁兰酶水解后,橡实粉丝具有极佳的质构特性。此外,酶解将橡实淀粉从表面光滑的球形颗粒转化为表面粗糙的多边形颗粒,并将橡实淀粉的晶体结构从 C 型转化为 B 型。与天然橡实淀粉相比,酶解橡实淀粉的直链淀粉含量更高,冻融稳定性更好,膨胀力、崩解黏度、凝胶强度更低,透光率更高。这些优良的特性有助于橡实粉丝的特殊质构和品质。本研究结果可为橡实粉丝的制备提供有价值的信息。

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