Fei Peng, Jing He, Ma Yan, Dong Gege, Chang Yunhe, Meng Zhaoxu, Jiang Shilong, Xie Qinggang, Li Shuzhen, Chen Xi, Yang Weiwei
School of Zhang Zhongjing Health Care and Food, Nanyang Institute of Technology, Nanyang, China.
Food and Pharmaceutical Engineering Institute, Guiyang University, Guiyang, China.
Front Microbiol. 2022 May 27;13:900690. doi: 10.3389/fmicb.2022.900690. eCollection 2022.
The purpose of this study was to investigate the prevalence of spp. in commercial powdered infant formula (PIF) from nine provinces in China from March 2018 to September 2020, and to reveal the genotype, biofilm-forming ability, and antibiotic susceptibility of these isolates. A total of 27 strains, consisting of 22 strains, 3 strains, 1 strain, and 1 strain, were isolated from 3,600 commercial PIF samples with a prevalence rate of 0.75%. Compared with the other 8 provinces, PIF from Shaanxi province had a higher prevalence rate (1.25%) of spp. These isolates were divided into 14 sequence types (STs), and 6 serotypes. The main STs were ST4, ST1, and ST64, and the dominant serotype was serotype O2. Approximately 88.89% of isolates had a strong ability (OD > 1) to form biofilms on tinplate, among which the strains with ST4 were more dominant. All isolates were susceptible to ampicillin-sulbactam, ceftriaxone, cefotaxime, sulfadiazine, sulfadoxine, trimethoprim-sulfamethoxazole, gentamicin, tetracycline, ciprofloxacin, and colistin, while 55.56 and 96.30% isolates were resistant to cephalothin and vancomycin, respectively. Taken together, our findings highlighted the contamination status and characterization of spp. in commercial PIF from nine provinces of China, and provided guidance for the effective prevention and control of this pathogen in the production of PIF.
本研究旨在调查2018年3月至2020年9月中国9个省份市售婴儿配方奶粉（PIF）中 spp. 的流行情况，并揭示这些分离株的基因型、生物膜形成能力和抗生素敏感性。从3600份市售PIF样本中分离出27株菌株，包括22株 菌株、3株 菌株、1株 菌株和1株 菌株，流行率为0.75%。与其他8个省份相比，陕西省PIF中 spp. 的流行率较高（1.25%）。这些分离株分为14个序列类型（STs）和6个血清型。主要的STs为ST4、ST1和ST64，优势血清型为O2血清型。约88.89%的 分离株在马口铁上具有较强的生物膜形成能力（OD>1），其中ST4菌株更为占主导。所有分离株对氨苄西林 - 舒巴坦、头孢曲松、头孢噻肟、磺胺嘧啶、磺胺多辛、甲氧苄啶 - 磺胺甲恶唑、庆大霉素、四环素、环丙沙星和黏菌素敏感，而分别有55.56%和96.30%的分离株对头孢噻吩和万古霉素耐药。综上所述，我们的研究结果突出了中国9个省份市售PIF中 spp. 的污染状况和特征，并为PIF生产中该病原体的有效预防和控制提供了指导。
