School of Biological Sciences and Biotechnology, Haramaya University, Oromia, Ethiopia.
Recent Pat Biotechnol. 2023;17(2):142-150. doi: 10.2174/1872208316666220617104318.
Lippia adoensis Hoechst var. adoensis (wild variety) and variety koseret (cultivated variety) have been used as traditional medicine, condiments, and endemic to Ethiopia.
This study aimed to assess the physicochemical properties and biological activities of oil extracts from seed and leaves of koseret (L. adoensis var. koseret).
Soxhlet apparatus was used for oil extraction using hexane as a solvent. The oil quality assessment was based on oil yield, acid value, percent free fatty acid, and peroxide value, while the biological activities were investigated based on antioxidant and antimicrobial activities. The antimicrobial experiment was arranged as 2 x1x4 in a completely randomized factorial design with three replications.
The result indicated that significantly higher oil yield (2.25%), acid value (2.66%) and free fatty acid (1.34%) were recorded for seed oil using the solvent extraction method. Leaf oil was recorded to have significantly higher values of DPPH (2,2- diphenyl-1- picrylhydrazyl), ascorbic acid and total carotenoid contents, but a lower value of hydrogen peroxide scavenging activity indicated that leaf oil presented higher antioxidant activity than seed oil in koseret. The koseret leaf oil demonstrated stronger antibacterial activity with a maximum zone of inhibition (14.50±0.21 mm), minimum inhibitory concentration (MIC, 0.25 μg/ml) and corresponding minimum bactericidal concentration (MBC, 0.25 μg/ml) against S. aureus. Furthermore, leaf oil has also presented stronger antifungal activity with a maximum zone of inhibition (14.83 mm), MIC (0.25 μg/ml), and minimum fungicidal concentration (MFC, 0.50 μg/ml) against Aspergillus versicolor.
It can be concluded from the results of this study that leaf oil extract has demonstrated better biological activities, including both antioxidant and antimicrobial potentials, than seed oil in koseret.
Lippia adoensis Hoechst var. adoensis（野生品种）和品种 koseret（栽培品种）已被用作传统药物、调味料，并在埃塞俄比亚特有。
本研究旨在评估 koseret（L. adoensis var. koseret）种子和叶片油提取物的理化性质和生物活性。
使用索氏提取器用正己烷作为溶剂提取油。根据油得率、酸值、游离脂肪酸百分比和过氧化物值评估油质，同时根据抗氧化和抗菌活性研究生物活性。抗菌实验采用完全随机因子设计，2 x1x4 排列，有三个重复。
结果表明，溶剂萃取法得到的种子油具有更高的产油率（2.25%）、酸值（2.66%）和游离脂肪酸（1.34%）。叶片油的 DPPH（2,2-二苯基-1-苦基肼）、抗坏血酸和总类胡萝卜素含量较高，但过氧化氢清除活性较低，表明 koseret 叶片油的抗氧化活性高于种子油。Koseret 叶片油对金黄色葡萄球菌的抑菌活性最强，抑菌圈最大（14.50±0.21mm），最小抑菌浓度（MIC，0.25μg/ml）和相应的最小杀菌浓度（MBC，0.25μg/ml）。此外，叶片油对黑曲霉也表现出较强的抗真菌活性，抑菌圈最大（14.83mm）、MIC（0.25μg/ml）和最低杀菌浓度（MFC，0.50μg/ml）。
从本研究结果可以得出结论，与 koseret 的种子油相比，叶片油提取物具有更好的生物活性，包括抗氧化和抗菌潜力。
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