MRC Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK.
Department of Critical Care Medicine, Safar Center for Resuscitation Research, Children's Neuroscience Institute, University of Pittsburgh, Pittsburgh, PA, USA.
Nat Metab. 2022 Jun;4(6):651-662. doi: 10.1038/s42255-022-00591-z. Epub 2022 Jun 27.
Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.
活性氧（ROS）的多种作用及其对健康和疾病的影响正在整个生命科学领域显现。这一发展促使不熟悉 ROS 复杂性及其反应的研究人员不恰当地使用商业试剂盒和探针来测量 ROS 和氧化损伤，将 ROS（通用缩写）视为离散的分子实体。不幸的是，这些测量的应用和解释充满了挑战和局限性。这可能导致误导性的主张进入文献，并阻碍进展，尽管已经有了关于如何最好地评估单个 ROS、它们的反应、作为信号分子的作用以及它们可能造成的氧化损伤的成熟知识体系。在这份共识声明中，我们阐明了在使用许多常用方法测量 ROS 和氧化损伤时可能出现的问题，并提出了最佳实践指南。我们希望这些策略对那些发现他们的研究需要评估细胞内和体内 ROS、氧化损伤和氧化还原信号的人有用。
