LncRNA PVT1 Regulates miR-1207-5p to Affect Colon Cancer Proliferation and Migration via the Wnt6/β-catenin2 Pathway.

We aimed to evaluate the effects of lncRNA PTV1 on colon cancer proliferation and migration via the Wnt6/β-catenin2 pathway. A total of 117 colon cancer and normal adjacent tissue samples were collected. and expressions in these samples and colon cancer cell lines were detected by Quantitative reverse transcription-polymerase chain reaction (qRT-PCR). -silencing cells and miR-1207-5p-overexpressing Caco-2-siPVT1 cells were constructed, respectively. The effects of silencing on cell proliferation were assessed by MTT and colony formation assays. The effects on invasion and migration were tested by Transwell and scratch assays respectively. The targeting regulatory relationship between and was analyzed by a dual-luciferase reporter assay. The relationship between and was studied by RNA-binding protein immunoprecipitation and RNA pull-down assays. The expressions of proteins in the Wnt6/β-catenin2 pathway were detected by Western blotting. The mRNA expression in colon cancer tissue was significantly higher than that in normal adjacent tissue ( < 0.05). The expression in -silencing cells was significantly down-regulated ( < 0.05). The colonies of Caco-2-siPVT1 cells decreased, accompanied by a reduced number of cells penetrating Matrigel and migration ( < 0.05). Compared with siPVT1 + NC group, the number of colonies and migration of siPVT1 + miR-1207-5p-overexpressing group increased significantly ( < 0.05). There was a targeting relationship between and . had a targeted binding site with . The protein expressions of Wnt6/β-catenin2 in Caco-2-siPVT1 group were significantly lower than those of control and Caco-2-siNC groups ( < 0.05). was highly expressed in colon cancer. It may enhance the proliferation and migration of colon cancer cells by up-regulating level and enhancing the Wnt6/β-catenin2 pathway.