由大肠杆菌质粒R6K编码的青霉素酶的部分氨基酸序列。
Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.
作者信息
Ambler R P, Scott G K
出版信息
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3732-6. doi: 10.1073/pnas.75.8.3732.
Abstract
Direct studies of the amino acid sequence of an Escherichia coli plasmid-coded penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) are in complete agreement with results derived from the translation of the DNA sequence of a related plasmid, apart from a single amino acid substitution. This penicillinase from a Gram-negative bacterium shows 30-35% identity with functionally similar enzymes from Gram-positive bacteria. This paper should be read in conjunction with the report of the DNA sequence of the gene.
摘要
对大肠杆菌质粒编码的青霉素酶（青霉素酰胺-β-内酰胺水解酶，EC 3.5.2.6）氨基酸序列的直接研究，除了一个氨基酸替换外，与源自相关质粒DNA序列翻译的结果完全一致。这种来自革兰氏阴性菌的青霉素酶与来自革兰氏阳性菌的功能相似酶有30%-35%的同源性。本文应与该基因DNA序列的报告一起阅读。
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