SIAISi021-A 细胞系的建立，源自一位 71 岁的患有阿尔茨海默病（AD）的中国汉族男性，他携带两份 APOE4/4 等位基因。
Establishment of SIAISi021-A, an induced pluripotent stem cell (iPSC) line from a 71-year-old Chinese Han male with Alzheimer's Disease (AD) having two copies of APOE4/4 allele.
机构信息
Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Neurology, Ruijin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech, China.
出版信息
Stem Cell Res. 2022 Aug;63:102866. doi: 10.1016/j.scr.2022.102866. Epub 2022 Jul 14.
A 71-year-old Han male from China contributed peripheral blood mononuclear cells (PBMCs). Non-integrative Sendai viral vectors containing reprogramming factors OCT4, KLF4, SOX2 and C-MYC were used to reprogram PBMCs. Pluripotency makers confirmed the pluripotency of transgene-free induced pluripotent stem cell (iPSC). The ability of iPSC to spontaneously differentiate three germ layers in vitro confirmed the pluripotency of iPSC. The iPSC line displayed a normal karyotype. The newly generated human iPSC SIAISi021-A can be used for studying further disease mechanisms of Alzheimer's Disease (AD).
一位 71 岁的中国汉族男性捐献了外周血单核细胞（PBMC）。使用非整合性仙台病毒载体将重编程因子 OCT4、KLF4、SOX2 和 C-MYC 转染到 PBMC 中。多能性标志物证实了无转基因的诱导多能干细胞（iPSC）的多能性。iPSC 能够在体外自发地分化为三个胚层，证实了 iPSC 的多能性。该 iPSC 系显示正常核型。新生成的人类 iPSC SIAISi021-A 可用于进一步研究阿尔茨海默病（AD）的疾病机制。
Zotero 插件