寒冷暴露诱导山羊棕色脂肪组织中的脂质动态和产热。
Cold exposure induces lipid dynamics and thermogenesis in brown adipose tissue of goats.
机构信息
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People's Republic of China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People's Republic of China.
出版信息
BMC Genomics. 2022 Jul 21;23(1):528. doi: 10.1186/s12864-022-08765-5.
BACKGROUND
Adaptive thermogenesis by brown adipose tissue (BAT) is important to the maintenance of temperature in newborn mammals. Cold exposure activates gene expression and lipid metabolism to provide energy for BAT thermogenesis. However, knowledge of BAT metabolism in large animals after cold exposure is still limited.
RESULTS
In this study, we found that cold exposure induced expression of BAT thermogenesis genes and increased the protein levels of UCP1 and PGC1α. Pathway analysis showed that cold exposure activated BAT metabolism, which involved in cGMP-PKG, TCA cycle, fatty acid elongation, and degradation pathways. These were accompanied by decreased triglyceride (TG) content and increased phosphatidylcholine (PC) and phosphatidylethanolamine (PE) content in BAT.
CONCLUSION
These results demonstrate that cold exposure induces metabolites involved in glycerolipids and glycerophospholipids metabolism in BAT. The present study provides evidence for lipid composition associated with adaptive thermogenesis in goat BAT and metabolism pathways regulated by cold exposure.
背景
棕色脂肪组织（BAT）的适应性产热对于新生哺乳动物体温的维持很重要。冷暴露会激活基因表达和脂质代谢，为 BAT 产热提供能量。然而，关于大型动物在冷暴露后 BAT 代谢的知识仍然有限。
结果
在这项研究中，我们发现冷暴露诱导了 BAT 产热基因的表达，并增加了 UCP1 和 PGC1α 的蛋白水平。通路分析表明，冷暴露激活了 BAT 代谢，涉及 cGMP-PKG、三羧酸循环、脂肪酸延长和降解途径。这伴随着 BAT 中甘油三酯（TG）含量的降低和甘油磷脂（PC 和 PE）含量的增加。
结论
这些结果表明，冷暴露诱导了 BAT 中甘油脂和甘油磷脂代谢相关的代谢物。本研究为山羊 BAT 中与适应性产热相关的脂质组成以及冷暴露调节的代谢途径提供了证据。
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