免疫磁珠筛选表达特定 PfEMP1 变异体的恶性疟原虫感染红细胞。

Immunomagnetic Selection of Plasmodium falciparum-Infected Erythrocytes Expressing Particular PfEMP1 Variants.

机构信息

Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

出版信息

Methods Mol Biol. 2022;2470:69-78. doi: 10.1007/978-1-0716-2189-9_7.

DOI:10.1007/978-1-0716-2189-9_7

PMID:35881339

Abstract

Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.

摘要

恶性疟原虫在感染的红细胞（IEs）表面表达广泛的蛋白质，包括恶性疟原虫红细胞膜蛋白 1（PfEMP1）家族的成员。本方案描述了一种使用 PfEMP1 特异性抗体进行免疫磁珠选择的方法，该方法可获得均匀表达特定 PfEMP1 蛋白的寄生虫克隆。随后通过流式细胞术检测相应 PfEMP1 的表达，选择的寄生虫可用于进一步分析。