College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
Molecules. 2022 Jul 13;27(14):4487. doi: 10.3390/molecules27144487.
Astilbin is a dihydroflavonol glycoside identified in many natural plants and functional food with promising biological activities which is used as an antioxidant in the pharmaceutical and food fields. This work investigated the interaction between astilbin and human serum albumin (HSA) and their effects on the antioxidant activity of astilbin by multi-spectroscopic and molecular modeling studies. The experimental results show that astilbin quenches the fluorescence emission of HSA through a static quenching mechanism. Astilbin and HSA prefer to bind at the Site Ⅰ position, which is mainly maintained by electrostatic force, hydrophobic and hydrogen bonding interactions. Multi-spectroscopic and MD results indicate that the secondary structure of HSA could be changed because of the interaction of astilbin with HSA. DPPH radical scavenging assay shows that the presence of HSA reduces the antioxidant capacity of astilbin. The explication of astilbin-HSA binding mechanism will provide insights into clinical use and resource development of astilbin in food and pharmaceutical industries.
紫云英苷是一种在许多天然植物和功能性食品中发现的二氢黄酮醇糖苷，具有有前途的生物活性，在医药和食品领域用作抗氧化剂。本工作通过多光谱和分子建模研究研究了紫云英苷与人血清白蛋白（HSA）之间的相互作用及其对紫云英苷抗氧化活性的影响。实验结果表明，紫云英苷通过静态猝灭机制猝灭 HSA 的荧光发射。紫云英苷和 HSA 优先结合在 Site Ⅰ 位置，主要通过静电力、疏水作用和氢键相互作用维持。多光谱和 MD 结果表明，由于紫云英苷与 HSA 的相互作用，HSA 的二级结构可能发生变化。DPPH 自由基清除实验表明，HSA 的存在降低了紫云英苷的抗氧化能力。紫云英苷-HSA 结合机制的阐释将为紫云英苷在食品和医药工业中的临床应用和资源开发提供依据。
