病例报告：柬埔寨国家疟疾监测计划检测到疟原虫 knowlesi。
Case Report: Cambodian National Malaria Surveillance Program Detection of Plasmodium knowlesi.
机构信息
Department of Critical Care Medicine, National Institutes of Health Clinical Center, Bethesda, Maryland.
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland.
出版信息
Am J Trop Med Hyg. 2022 Jul 13;107(1):151-153. doi: 10.4269/ajtmh.22-0039.
Abstract
Despite recent success in reducing the regional incidence of Plasmodium falciparum malaria, cases of zoonotic malaria are on the rise in Southeast Asia. The Cambodian National Malaria Surveillance Program has previously relied on rapid diagnostic tests and blood smear microscopy with confirmatory polymerase chain reaction (PCR) testing in a subset of cases to further distinguish P. falciparum, P. malariae, P. ovale, and P. vivax species. Here, metagenomic next-generation sequencing identified P. knowlesi mono-infection in six Cambodian patients initially diagnosed with P. malariae by blood smear microscopy in February-May 2020. These findings of recent human infections with P. knowlesi in Cambodia led to the incorporation of P. knowlesi-specific PCR diagnostics to national malaria surveillance efforts.
摘要
尽管最近在降低恶性疟原虫疟疾的区域发病率方面取得了成功，但东南亚的人畜共患疟疾病例正在上升。柬埔寨国家疟疾监测计划以前依赖于快速诊断测试和血涂片显微镜检查，并对一部分病例进行聚合酶链反应（PCR）确认测试，以进一步区分恶性疟原虫、间日疟原虫、卵形疟原虫和三日疟原虫。在这里，宏基因组下一代测序鉴定出 6 例柬埔寨患者为诺氏疟原虫单感染，这些患者最初在 2020 年 2 月至 5 月通过血涂片显微镜检查被诊断为间日疟原虫。这些柬埔寨近期人类感染诺氏疟原虫的发现导致了将诺氏疟原虫特异性 PCR 诊断方法纳入国家疟疾监测工作中。
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