番鸭血管生成素样蛋白 4 基因的分子特征、时空表达及其与脂肪沉积的关系。
Molecular characterisation, temporal expression and involvement of ANGPTL4 in fat deposition in Muscovy ducks.
机构信息
College of Animal Science, Zhejiang University, Hangzhou, Zhejiang, China.
Ministry of Education, Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Zhejiang, China.
出版信息
Br Poult Sci. 2022 Dec;63(6):795-803. doi: 10.1080/00071668.2022.2102888. Epub 2022 Aug 31.
Abstract
- Angiopoietin-like protein 4 (ANGPTL4) plays a key role in promoting fat metabolism and reducing blood lipid. This study characterised the gene in Muscovy ducks () and investigated its potential functions in fat metabolism in Muscovy ducks.2. The Muscovy duck was successfully cloned for the first time, showing a length of 2591 bp. After phylogenetic analysis by RT-qPCR analysis, it was found that the gene of shared the highest sequence similarity with that of Muscovy ducks.3. The gene was commonly expressed in 23 different tissues with the highest expression in the abdominal fat in Muscovy ducks. A significant association was observed between abdominal fat percentage (AFP) and the expression of the gene. Moreover, Muscovy ducks showed a peak of expression in the liver and abdominal fat at 1-2 weeks old.4. These findings suggest that the gene is related to fat deposition and regulation in Muscovy ducks.
摘要
血管生成素样蛋白 4（ANGPTL4）在促进脂肪代谢和降低血脂方面发挥着关键作用。本研究对番鸭（Muscovy ducks）中的该基因进行了特征描述，并探讨了其在番鸭脂肪代谢中的潜在功能。
首次成功克隆了番鸭，其长度为 2591bp。通过 RT-qPCR 分析进行系统发育分析后发现，番鸭的基因与番鸭的基因具有最高的序列相似性。
该基因在 23 种不同组织中普遍表达，在番鸭的腹部脂肪中表达量最高。番鸭的腹部脂肪百分比（AFP）与基因的表达呈显著正相关。此外，番鸭在 1-2 周龄时肝脏和腹部脂肪中的表达量达到峰值。
这些发现表明，该基因与番鸭的脂肪沉积和调节有关。
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