College of Agronomy, Inner Mongolia Agricultural University, No. 275, XinJian EastStreet, Hohhot, 010019, China.
Sci Rep. 2022 Aug 5;12(1):13478. doi: 10.1038/s41598-022-16971-0.
Drought stress and the scarcity of nitrogen fertilizer are two of the important abiotic factors affecting maize growth. Bio-char can enhance the maize yield. Therefore, two field experiments were carried out in the 2 years (2019-2020) to study the effects of nitrogen fertilizer at three levels and four levels of bio-char on endogenous protective enzymes, dry matter accumulation, and yield of the maize 'Xianyu 335' under two different irrigation methods. A split-plot system in three replications was established to conduct the field trials. Two irrigation methods (Regular irrigation and Irregular irrigation) were in the main plots, three nitrogen fertilization levels (0, 150, 300 kg h m) were in sub-plots, and four bio-char levels (0, 8, 16, 24 t h m) were in the sub-sub plots. Each sub-plot consisted of 9 rows with 5 m length and 0.6 m width, and each sub-plot area was 30 m in the 2 years. The results indicated that the irrigation methods, the nitrogen, and bio-char supply significantly affected the maize endogenous protective enzymes, dry matter accumulation, and yield in the 2 years. Under the same irrigation method, nitrogen fertilizer and bio-char significantly improved the endogenous protective enzyme activity, dry matter accumulation, and yield of maize compared to the treatment without nitrogen fertilizer and bio-char. The above characteristics improved with increased bio-char supply and nitrogen fertilization at 150 kg h m. The treatment of C24N150 recorded the highest values for the parameters of maize endogenous protective enzymes activity, dry matter accumulation, and yield under different irrigation methods during the two harvest seasons.
干旱胁迫和氮素肥料缺乏是影响玉米生长的两个重要非生物因素。生物炭可以提高玉米产量。因此，在 2019-2020 年的两年中进行了两项田间试验，以研究在两种不同灌溉方式下，三个氮素水平和四个生物炭水平对玉米‘先玉 335’内源保护酶、干物质积累和产量的影响。采用三重复裂区设计进行田间试验。两个灌溉方式（常规灌溉和非规律灌溉）为主区，三个氮素水平（0、150、300kg hm）为副区，四个生物炭水平（0、8、16、24thm）为副副区。每个副区由 9 行组成，行长 5m，宽 0.6m，两年每个副区面积为 30m。结果表明，灌溉方式、氮素和生物炭供应在两年内显著影响玉米内源保护酶、干物质积累和产量。在相同的灌溉方式下，与不施氮和生物炭的处理相比，氮素和生物炭显著提高了玉米内源保护酶活性、干物质积累和产量。随着生物炭供应和 150kg hm 的氮素施肥量的增加，上述特性得到改善。在不同灌溉方式下，C24N150 处理在两个收获季节的玉米内源保护酶活性、干物质积累和产量参数中均表现出最高值。
