Hepatitis Virus Diversity Research Unit, Department of Internal Medicine, School of Clinical Medicine, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Life Sciences Imaging Facility, University of the Witwatersrand, Johannesburg, South Africa.
Front Cell Infect Microbiol. 2022 Jul 22;12:794264. doi: 10.3389/fcimb.2022.794264. eCollection 2022.
The world has seen the emergence of a new virus in 2019, SARS-CoV-2, causing the COVID-19 pandemic and millions of deaths worldwide. Microscopy can be much more informative than conventional detection methods such as RT-PCR. This review aims to present the up-to-date microscopy observations in patients, the studies of the virus and viral proteins and their interaction with their host, discuss the microscopy techniques for detection and study of SARS-CoV-2, and summarize the reagents used for SARS-CoV-2 detection. From basic fluorescence microscopy to high resolution techniques and combined technologies, this article shows the power and the potential of microscopy techniques, especially in the field of virology.
2019 年，世界出现了一种新型病毒，即 SARS-CoV-2，导致了 COVID-19 大流行和全球数百万人死亡。显微镜检查比 RT-PCR 等传统检测方法能提供更多信息。本综述旨在介绍目前在患者、病毒及其与宿主相互作用的研究方面的显微镜观察结果，讨论用于检测和研究 SARS-CoV-2 的显微镜技术，并总结用于 SARS-CoV-2 检测的试剂。从基础荧光显微镜到高分辨率技术和组合技术，本文展示了显微镜技术的力量和潜力，尤其是在病毒学领域。
Zotero 插件
