Characterization of Chitin Synthase A cDNA from (Hemiptera: Liviidae) and Its Response to Diflubenzuron.

Kuwayama is the vector of HLB and one of the most common pests in citrus orchards in southern China. One of the most significant genes in 's growth and development is the chitin synthase gene. In this study, the CHS gene () of was cloned and analyzed by bioinformatics. According to RT-qPCR findings, was expressed at many growth processes of , with the greatest influence in the fifth-instar nymph. The molting failure rate and mortality of rose as DFB concentration increased in this research, as did the expression level of . Feeding on caused a large drop in target gene expression, affected nymph molting, caused failure or even death in freshly eclosion adults, increased mortality, and reduced the molting success rate over time. These findings showed that was involved in nymph to adult development and may aid in the identification of molecular targets for regulation. It provided new ideas for further control of .